Autor(es)

Pieretti, Joana Claudio ; Armentano, Giovana Marchini ; Carneiro-Ramos, Marcela Sorelli ; Seabra, Amedea Barozzi

• Tipo de documento: Artigo Científico
• Fonte: NITRIC OXIDE-BIOLOGY AND CHEMISTRY; v. 158, p. 8-pg., 2025-10-01.

Resumo

Modulating intracellular nitric oxide (NO) levels offers a promising strategy to enhance tumor cell sensitivity to nanoparticle-based therapies. In this study, we investigated the impact of intracellular NO modulation in prostate cancer cells (PC3) using S-nitrosoglutathione (GSNO, a NO donor) and L-nitro arginine methyl ester (L-NAME, a nitric oxide synthase (NOS) inhibitor), in combination with cisplatin-loaded zinc oxide nanoparticles (ZnO/CisPt NPs). These nanoparticles, previously shown to exert selective cytotoxicity against PC3 cells, had their therapeutic performance further enhanced by NO modulation, which led to reduced NOS expression and regulation of inflammatory cytokines. Interestingly, both the increase and the depletion of intracellular S-NO levels contributed to tumor cell sensitization to the nanoparticle-based treatment. These results indicate that altering NO balance, regardless of direction, plays a key role in how cells respond to therapy. Our results reinforce the relevance of NO signaling in augmenting the efficacy of nanomedicine approaches while minimizing tumorassociated inflammation, offering a safer and more targeted strategy for prostate cancer treatment with potential for broader applications in oncology. (AU)

• Processo FAPESP: 22/14645-2 - Hidrogéis e próteses vasculares liberadores de óxido nítrico para aplicações cardiovasculares
• Beneficiário: Marcelo Ganzarolli de Oliveira
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 20/03646-2 - Impacto das nanoplataformas baseadas em óxido nítrico e quimioterápicos na citotoxicidade e sensibilização de células tumorais resistentes
• Beneficiário: Joana Claudio Pieretti
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 22/01124-4 - Impacto da resposta inflamatória em células cardíacas e renais induzido por proteínas acessórias do SARS-CoV-2: estudo das proteínas ORF 3a, ORF 6 e ORF 8
• Beneficiário: Giovana Marchini Armentano
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 24/12112-2 - Nanopartículas aliadas a transmissores gasosos para aplicações biomédicas e agrícolas
• Beneficiário: Amedea Barozzi Seabra
• Modalidade de apoio: Auxílio à Pesquisa - Regular