| Texto completo | |
| Autor(es): |
Lima, Tayna E.
;
Brito, Carlos A. A.
;
Gil, Laura H. V. G.
;
Dhalia, Rafael
;
Teixeira, Franciane M. E.
;
Sato, Maria N.
;
Wallau, Gabriel L.
;
Lins, Roberto D.
;
Viana, Isabelle F. T.
Número total de Autores: 9
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Journal of Clinical Virology; v. 181, p. 4-pg., 2025-12-25. |
| Resumo | |
The Coronavirus disease 2019 (COVID-19) pandemic has raised concerns regarding the specificity of serological assays due to potential antibody cross-reactivity. In this study, we analyzed sera from 57 individuals infected with the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) using enzyme-linked immunosorbent assay (ELISA) to assess antibody recognition of a human immunodeficiency virus type 1 (HIV-1) envelope epitope (2F5). Cross-reactive binding to the HIV-1 antigen was detected in 38.5 % (22/57) of samples, indicating nonspecific antibody recognition. Interestingly, sera from HIV-1-infected individuals and the monoclonal anti-2F5 antibody exhibited no reactivity toward the SARS-CoV-2 Spike receptor-binding domain (RBD), indicating that cross-reactivity occurs in a unidirectional manner and does not compromise the specificity of RBD-based diagnostic assays. These findings support the specificity of RBD-targeted assays and advocate for careful interpretation of HIV-1 serological assays in individuals with recent SARS-CoV-2 infection, particularly where dual circulation occurs. (AU) | |
| Processo FAPESP: | 23/11479-7 - Protocolo vacinal em modelo murino, como estratégia de proteção à infecção por Zika Vírus |
| Beneficiário: | Franciane Mouradian Emidio Teixeira |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |
| Processo FAPESP: | 19/25119-7 - Interface materno-fetal: imunopatogênese e intervenção vacinal em infecções virais |
| Beneficiário: | Maria Notomi Sato |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |