| Texto completo | |
| Autor(es): |
Chaves, Matheus Andrade
;
Prado, Cibele Maria
;
Fiorenza, Mariani Farias
;
Almeida, Maria Alice de
;
Souza, Camila Azzolin de
;
Perecin, Felipe
;
Pinho, Samantha Cristina de
;
Silveira, Juliano Coelho da
Número total de Autores: 8
|
| Tipo de documento: | Artigo Científico |
| Fonte: | COLLOIDS AND SURFACES B-BIOINTERFACES; v. 257, p. 13-pg., 2026-01-26. |
| Resumo | |
The development of liposomal formulations tailored for interaction with granulosa cells holds significant promise for applications in reproductive biotechnology. In this study, both anionic and cationic liposomes were formulated using purified and nonpurified phosphatidylcholines and produced using probe sonication followed by extrusion. Liposomes were characterized for size, polydispersity index (PDI), and surface charge, and evaluated for morphology, cytotoxicity, internalization efficiency, and effects on gene expression. Dynamic light scattering revealed nanometric sizes for both formulations (122.1 +/- 2.1 nm for anionic and 119.4 +/- 2.7 nm for cationic, respectively), with low PDI values (< 0.2), and stable zeta potentials (-31.7 +/- 1.4 mV and 48.3 +/- 2.6 mV, respectively). Morphology analyses confirmed spherical/near-spherical morphology. Cytotoxicity assays demonstrated that anionic liposomes were biocompatible across all tested concentrations, whereas cationic liposomes induced a dose-dependent effect. Fluorescence microscopy revealed efficient uptake of both liposomal types by granulosa cells, with cationic liposomes exhibiting intracellular fluorescence up to 12 h. Gene expression analysis targeting lipid metabolism, oxidative stress response, steroidogenesis, and apoptosis regulation genes indicated that neither liposomal formulation induced detrimental shifts in cellular homeostasis in the analyzed period. In conclusion, the findings support the use of differently charged liposomal formulations as promising platforms for intracellular delivery in cell-based applications, offering potential for future strategies involving the delivery of genetic material and subsequent modulation of cellular functions. (AU) | |
| Processo FAPESP: | 22/01505-8 - EMU concedido no processo FAPESP 21/06645-0 : CytoFLEX System B3-R0-V3 |
| Beneficiário: | Juliano Coelho da Silveira |
| Modalidade de apoio: | Auxílio à Pesquisa - Programa Equipamentos Multiusuários |
| Processo FAPESP: | 14/22887-0 - Exossomos e microvesículas contendo miRNAs modulam mudanças epigenéticas durante o cultivo in vitro de gametas e embriões em bovinos |
| Beneficiário: | Juliano Coelho da Silveira |
| Modalidade de apoio: | Auxílio à Pesquisa - Jovens Pesquisadores |
| Processo FAPESP: | 21/06645-0 - Vesículas extracelulares como uma plataforma para diagnóstico e manipulação do sistema de produção in vitro de embriões: a nova geração da reprodução animal |
| Beneficiário: | Juliano Coelho da Silveira |
| Modalidade de apoio: | Auxílio à Pesquisa - Jovens Pesquisadores - Fase 2 |
| Processo FAPESP: | 15/21829-9 - EMU concedido no projeto 2014/22887-0: NanoSight LM10 |
| Beneficiário: | Juliano Coelho da Silveira |
| Modalidade de apoio: | Auxílio à Pesquisa - Programa Equipamentos Multiusuários |
| Processo FAPESP: | 22/01236-7 - Vesículas híbridas e sintéticas formadas por vesículas extracelulares e lipossomas para a suplementação de células embrionárias durante a produção in vitro de bovinos |
| Beneficiário: | Matheus Andrade Chaves |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |