| Texto completo | |
| Autor(es): Mostrar menos - |
Doimo, Ana L. C.
;
De Annunzio, Sarah R.
;
Fragelli, Bruna D. L.
;
Ribeiro, Lara K.
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Oliveira, Marisa C.
;
Ribeiro, Renan A. P.
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Teodoro, Marcio D.
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Moraes, Bruna L.
;
Barbugli, Paula A.
;
Vergani, Carlos E.
;
Anibal, Fernanda F.
;
Longo, Elson
;
Assis, Marcelo
Número total de Autores: 13
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| Tipo de documento: | Artigo Científico |
| Fonte: | SURFACES AND INTERFACES; v. 76, p. 14-pg., 2025-10-16. |
| Resumo | |
Silver silicate nanoparticles were synthesized via co-precipitation followed by microwave-assisted hydrothermal treatment for 0-64 min. X-ray diffraction confirmed that all samples were amorphous, while infrared spectroscopy, photoluminescence, and selected area electron diffraction revealed progressive short- and medium-range structural rearrangements with increasing irradiation time. These changes led to the formation of semi-crystalline domains and were accompanied by the evolution of structural defects, particularly oxygen vacancies. Transmission electron microscopy showed a reduction in particle size attributed to dissolution-recrystallization dynamics under microwave exposure. Elemental analyses indicated a progressive incorporation of silver, with the Ag/Si atomic ratio shifting from similar to 1:3 to nearly 1:1. Antimicrobial assays demonstrated enhanced activity with longer synthesis times; the 64 min sample showed the lowest inhibitory and bactericidal concentrations against Staphylococcus aureus, Escherichia coli, and Candida albicans. Cytotoxicity tests using murine fibroblasts NIH/3T3 confirmed that the effective antimicrobial concentrations remained below toxicity thresholds. The production of reactive oxygen species, including hydroxyl radicals and singlet oxygen, was experimentally verified using specific molecular probes and increased with longer irradiation times. These findings were supported by theoretical calculations, which demonstrated the role of the amorphous surface in promoting water and oxygen adsorption, enabling reactive oxygen species formation. Overall, this work shows that microwave-assisted synthesis enables precise tuning of structure, composition, and defect profiles in amorphous silver silicate, resulting in a material with strong antimicrobial performance and favorable biological compatibility. (AU) | |
| Processo FAPESP: | 23/10462-3 - Modificação de Silicato de Prata com Feixe de Elétrons para uma Eficácia Superior na Eliminação de Patógenos |
| Beneficiário: | Ana Luiza de Camargo Doimo |
| Modalidade de apoio: | Bolsas no Brasil - Mestrado |
| Processo FAPESP: | 23/08525-7 - Desenvolvimento de curativos cutâneos 3D de colágeno marinho enriquecidos com nanoparticulas funcionais para a regeneração de feridas cutâneas |
| Beneficiário: | Marcelo de Assis |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |
| Processo FAPESP: | 22/08487-5 - Propriedades antimicrobianas e biocompatibilidade de titânio e zircônia revestidos com ±-Ag2WO4 irradiado com feixe de elétrons |
| Beneficiário: | Sarah Raquel de Annunzio |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |
| Processo FAPESP: | 21/01651-1 - CDMF - Centro de Desenvolvimento de Materiais Funcionais: estudo teórico de materiais multifuncionais com potencial aplicação antiviral |
| Beneficiário: | Marisa Carvalho de Oliveira |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |
| Processo FAPESP: | 22/06210-6 - Efeito antitumoral de diferentes morfologias de semicondutores a base de Ag contra células tumorais de bexiga |
| Beneficiário: | Bruna Dias de Lima Fragelli |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |