| Texto completo | |
| Autor(es): |
Carriero, Mateus Maldonado
;
Henrique-Silva, Flavio
;
Meira, Caroline Munhoz
;
Queiroz Gato, Igor Mateus
;
Caetano, Alexandre Rodrigues
;
Lobo, Francisco Pereira
;
Alves, Anderson Luis
;
Varela, Eduardo Sousa
;
Mendes Maia, Antonio Augusto
Número total de Autores: 9
|
| Tipo de documento: | Artigo Científico |
| Fonte: | GENETICS AND MOLECULAR BIOLOGY; v. 43, n. 4, p. 15-pg., 2020-01-01. |
| Resumo | |
In the present study, the complete characterization of cDNA and genomic sequences of IL-1 beta and IL-8, as well as the expression profile of these genes in the South American fish pacu (Piaractus mesopotamicus) is provided. The full-length pmIL-1 beta cDNA was composed of 1208 nucleotides that would produce a precursor peptide with 273 amino acid residues. A putative caspase-1 cleavage site, similar to what is found in mammalian IL-1 beta, was identified producing a mature peptide with a theoretical molecular weight of 17.21 kDa. The pmIL-8 cDNA sequence consisted of 1019 nucleotides which encoded a 95-amino acid protein with a theoretical molecular weight of 10.43 kDa that showed all typical CXC chemokine features, including a 20-residue signal peptide and four conserved cysteine residues. Constitutive m RNA expression was detected for both genes in the liver, head kidney, gill, intestine, skin and spleen. After a bacterial challenge, up-regulation was detected for both pmIL-1 beta and pmIL-8 in the spleen and head kidney at 12 h post-infection. At 24 h post-infection there was a decrease in the expression of both genes, with pm IL-8 showing a significant down-regulation in the liver and head kidney when compared to the control groups. (AU) | |
| Processo FAPESP: | 17/18562-6 - Imunização de lambaris (Astyanax altiparanae) com vacina gênica contendo o antígeno de imobilização IAG52A de Ichthyophthirius multifiliis em conjunto com a citocina IL-8 de A. altiparanae como adjuvante molecular |
| Beneficiário: | Mateus Maldonado Carriero |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |
| Processo FAPESP: | 17/26996-6 - Avaliação da triploidia na susceptibilidade à infecção por Aeromonas jandaei e possível relação com os níveis de expressão de genes de resposta imune em lambaris (Astyanax altiparanae) |
| Beneficiário: | Antonio Augusto Mendes Maia |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |