| Texto completo | |
| Autor(es): |
Roberto, Gabbai-Armelin Paulo
;
Analu Barros, De Oliveira
;
Fernanda, Rodero Camila
;
Miguel, Silva Jhonatan
;
Aparecida Rodrigues, Fernandes Natalie
;
Robert, Paiva
;
Cristina, Dametto Alessandra
;
Marlus, Chorilli
;
Hernane Da Silva, Barud
;
Lourencao, Brighenti Fernanda
Número total de Autores: 10
|
| Tipo de documento: | Artigo Científico |
| Fonte: | CURRENT TOPICS IN MEDICINAL CHEMISTRY; v. N/A, p. 16-pg., 2025-10-17. |
| Resumo | |
Introduction Skin wounds represent a worldwide problem. Biopolymers have been attracting interest in healthcare products for wound dressing. Among these, bacterial nanocellulose membranes (BNC) are attractive for their unique structure, but they lack antimicrobial activity. Thus, the incorporation of the monoterpenes Carvacrol (Car) and Thymol (Thy) - which present antimicrobial and healing properties - toward the improvement of skin wound healing, consists of an appealing approach. This research aimed to produce and characterize nanocellulose membranes containing carvacrol and/or thymol, and investigate their release behavior, cytotoxicity, and antimicrobial properties.Method BNC/Car, BNC/Thy, and BNC/Car-Thy membranes were produced at doses of 0.1 and 1.0 mg/cm2.Results The natural components incorporation into the nanocellulose did not interfere with the ultra-structure or its physical characteristics. Pilot studies showed that membranes with 1.0 mg/cm2 of monoterpenes were toxic to fibroblasts. Therefore, all further studies used the lower dose of 0.1 mg/cm2. Release experiments showed a burst release between 2-4 h with sustained release till 24 h, reaching around 80% of the initial amount of the incorporated monoterpenes. Studies with fibroblast and keratinocytes indicated no cytotoxicity and that cells could proliferate over the BNC/Car-Thy membranes. Microbiological studies suggested some antimicrobial potential of the BNC doped with Car and Thy.Discussion BNC membranes incorporated with Car and Thy were successfully produced and the monoterpenes incorporation into the BNC did not interfere with either ultra-structure or with its physico-chemical characteristics. Natural products incorporation induced cell proliferation and presented antimicrobial properties, besides increasing the solubility and stability of these natural compounds.Conclusion This innovative biomaterial has the potential for healthcare products. (AU) | |
| Processo FAPESP: | 19/08375-0 - Potencialização do efeito anticárie e antibiofilme de substâncias convencionais: avaliação em modelo de biofilme polimicrobiano |
| Beneficiário: | Fernanda Lourenção Brighenti |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 13/07793-6 - CEPIV - Centro de Ensino, Pesquisa e Inovação em Vidros |
| Beneficiário: | Edgar Dutra Zanotto |
| Modalidade de apoio: | Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs |
| Processo FAPESP: | 20/07110-0 - Potencial da terapia fotodinâmica na modulação da resposta inflamatória e nos fatores de virulência microbianos: avaliação em modelo de co-culturas tridimensionais de fibroblastos gengivais, Candida albicans e Staphylococcus aureus |
| Beneficiário: | Analú Barros de Oliveira |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |
| Processo FAPESP: | 17/50334-3 - Plano de Desenvolvimento Institucional em Pesquisa (PDIp): modernização e adequação de unidades multiusuárias estratégicas do Instituto Biológico |
| Beneficiário: | Ana Eugênia de Carvalho Campos |
| Modalidade de apoio: | Auxílio à Pesquisa - Programa Modernização de Institutos Estaduais de Pesquisa |
| Processo FAPESP: | 18/25512-8 - Plataforma para Cultura de Células Baseada em Biocelulose |
| Beneficiário: | Hernane da Silva Barud |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |