5-Aminosalicylic Acid (5-ASA) Can Reduce Levels of Oxidative DNA Damage in Cells of Colonic Mucosa With and Without Fecal Stream

Autor(es)

Caltabiano, Caroline ^[1] ; Maximo, Felipe Rodrigues ^[2] ; Spadari, Ana Paula Pimentel ^[2] ; da Conceicao Miranda, Daniel Duarte ; Pivatto Serra, Marcia Milena ^[1] ; Ribeiro, Marcelo Lima ^[1] ; Real Martinez, Carlos Augusto ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Francisco, Sch Med, Postgrad Program Hlth Sci, BR-12900000 Braganca Paulista, SP - Brazil

^[2] Univ Sao Francisco, Hosp Med, BR-12900000 Braganca Paulista, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Digestive Diseases and Sciences; v. 56, n. 4, p. 1037-1046, APR 2011.
• Citações Web of Science: 13

Resumo

No studies have evaluated the effectiveness of 5-ASA against oxidative DNA damage in experimental models of diversion colitis. To evaluate the effects of 5-ASA against oxidative DNA damage in an experimental model of diversion colitis. Twenty-six Wistar rats were divided into two groups corresponding to sacrifice at 2 or 4 weeks after fecal diversion of the left colon by means of proximal colostomy and distal mucosa fistula. Each group was divided into two subgroups according to intervention in excluded colon performed with 0.9% saline solution or 5-ASA. Level of oxidative DNA damage was determined by comet assay in cells obtained from segments with and without fecal stream before and after H(2)O(2) challenge. For statistical analysis, was used one-way analysis of variance (ANOVA), adopting a 5% significance level (P < 0.05). Levels of DNA damage were always higher in colon segments without fecal stream, regardless of the intervention solution employed. DNA damage in colon segments with and without fecal stream in animals irrigated with 5-ASA was lower when compared with those treated with saline solution, regardless of time of irrigation. These levels remained lower after intervention with 5-ASA, even after H(2)O(2) challenge. Enema with 5-ASA reduces oxidative DNA damage in epithelial cells of colon segments without fecal stream, even after H(2)O(2) challenge, confirming the effects of 5-ASA against DNA damage by oxygen free radicals. (AU)

• Processo FAPESP: 06/02306-6 - Avaliação da correlação entre as alterações histológicas, expressão tecidual da proteína p53 e o dano oxidativo do DNA nas células da mucosa cólica de ratos submetidos a colite de exclusão
• Beneficiário: Carlos Augusto Real Martinez
• Modalidade de apoio: Auxílio à Pesquisa - Regular