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Advances in the Biosynthesis of Pyranocoumarins: Isolation and 13C-Incorporation Analysis by High-Performance Liquid Chromatography-Ultraviolet-Solid-Phase Extraction-Nuclear Magnetic Resonance Data

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Amaral, Jessica C. ; da Silva, Michelli M. ; da Silva, M. Fatima G. F. ; Alves, Thayana C. ; Ferreira, A. Gilberto ; Forim, Moacir R. ; Fernandes, Joao B. ; Pina, Edieidia S. ; Lopes, Adriana A. ; Pereira, Ana M. S. ; Novelli, Valdenice M.
Número total de Autores: 11
Tipo de documento: Artigo Científico
Fonte: Journal of Natural Products; v. 83, n. 5, p. 7-pg., 2020-05-22.
Resumo

Citrus sinensis and Citrus limonia were obtained by germination from seeds, and isotopic-labeling experiments using D-[1-C-13]glucose were performed with the seedlings. After 60 days, the seedlings were analyzed by high-performance liquid chromatography-ultraviolet-solid-phase extraction-nuclear magnetic resonance, data and the C-13 enrichment patterns of xanthyletin and seselin indicated that the pyran ring was formed by the methylerythritol phosphate pathway and that the coumarin moiety was derived from the shikimate pathway in both compounds. This information regarding the biosynthetic pathway can be used to increase resistance against phytopathogens, because xanthyletin and seselin are reported to have antimicrobial activity on the growth of Xylella fastidiosa, which causes citrus variegated chlorosis in orange. (AU)

Processo FAPESP: 14/50249-8 - Green chemistry: sustainable synthetic methods employing benign solvents, safer reagents, and bio-renewable feedstock
Beneficiário:Arlene Gonçalves Corrêa
Modalidade de apoio: Auxílio à Pesquisa - Programa Centros de Pesquisa Aplicada
Processo FAPESP: 12/25299-6 - Estudos integrados para o controle de formigas cortadeiras
Beneficiário:João Batista Fernandes
Modalidade de apoio: Auxílio à Pesquisa - Temático
Processo FAPESP: 14/50918-7 - INCT 2014: para o Controle Biorracional de Insetos Pragas e Fitopatógenos
Beneficiário:Maria Fátima das Graças Fernandes da Silva
Modalidade de apoio: Auxílio à Pesquisa - Temático