| Texto completo | |
| Autor(es): |
Birolli, Willian G.
;
Porto, Andre L. M.
;
Fonseca, Luis P.
Número total de Autores: 3
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Bioresource Technology; v. 297, p. 15-pg., 2020-02-01. |
| Resumo | |
Miniemulsion systems presented a great potential for biocatalytic reactions. However, different limitations jeopardized the applications of this non-conventional medium. In this work, miniemulsion systems (emulsion reactors) were applied for the first time to the aldol reaction between cyclohexanone and 4-nitrobenzaldehyde by Rhizopus niveus lipase allowing a decrease in the catalyst concentration from 20 mg mL(-1) to 6 mg mL(-1) in comparison with organic solvents. Moreover, the yield increased from similar to 25% to similar to 65% for 48 h reactions and the enantiomeric excess increased from similar to 10% to similar to 30% for (R,S)-anti-aldol product, showing the potentiality of this non-conventional reaction medium. The advances reported in this work expands the possibilities of the miniemulsion reaction medium to a whole new level, increasing the scope to solid reagents and products, and also different reactions (biocatalytic or not) that requires pH control by buffers with a simple product isolation procedure, enabling future applications of this poorly studied reaction medium. (AU) | |
| Processo FAPESP: | 14/18257-0 - Novas abordagens em reações biocatalíticas |
| Beneficiário: | Andre Luiz Meleiro Porto |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |