| Texto completo | |
| Autor(es): |
Bravo, Maria da Graca Landim
;
da Silva, Isadora de Jesus
;
de Araujo, Nayara Fernanda Tokashike
;
Cezarette, Gabriel Neves
;
Tonani, Ludmilla
;
Kress, Marcia Regina von Zeska
;
de Vecchi, Rodrigo
;
Dakic, Vanja
;
Gaspar, Lorena Rigo
Número total de Autores: 9
|
| Tipo de documento: | Artigo Científico |
| Fonte: | International Journal of Pharmaceutics; v. 689, p. 12-pg., 2025-12-19. |
| Resumo | |
The Reconstructed Human Epidermis (RHE) model offers a significant in vitro alternative to animal testing for cosmetic safety and efficacy. This physiologically relevant system evaluates skin responses, meeting ethical, scientific, and regulatory demands. It is particularly useful for studying ultraviolet radiation (UV) and air pollution, key stressors causing oxidative stress and inflammation. This study aimed to optimize a robust photopollution model using the SkinEthicTM RHE, demonstrating its applicability for evaluating oxidative stress biomarkers and antioxidant protection under combined UVA and airborne particulate matter (PM) exposure. RHE models were exposed to nebulized PM2.5 (100 mu g/mL; deposited dose: 9 mu g/cm2), followed by UVA radiation (6 J/cm2). ROS were quantified using the DCFH2-DA (dichlorodihydrofluorescein diacetate) probe. Exposure to PM2.5 alone did not increase ROS generation, whereas UVA exposure alone induced a 45 % rise in intracellular ROS compared to the negative control. Co-exposure to PM2.5 and UVA produced a significantly higher ROS level (95 % increase), demonstrating a synergistic effect of the combined stressors. Assessments on oxidative stress biomarkers were performed at two key time points: 4 h post-exposure, capturing both short-term gene expression patterns, and 24 h post-exposure, for inflammatory cytokine release. Co-exposure significantly amplified NRF2 and IL-18 gene upregulation and induced significant IL-18 secretion. IL-18 was identified as the most responsive inflammatory marker under the tested conditions. Overall, the present RHE exposure model demonstrated predictive capacity for investigating photopollution-induced oxidative stress and some of its related biomarkers, supporting its further applicability in studying skin-environment interactions. (AU) | |
| Processo FAPESP: | 14/50928-2 - INCT 2014: Nanotecnologia Farmacêutica: uma abordagem transdisciplinar |
| Beneficiário: | Maria Vitória Lopes Badra Bentley |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 21/06619-9 - Modelos in vitro fisiologicamente relevantes para explorar novos compostos com atividade protetora contra a radiação UV e poluição ambiental |
| Beneficiário: | Lorena Rigo Gaspar Cordeiro |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |