| Texto completo | |
| Autor(es): |
Annes, Kelly
;
Sudano, Mateus Jose
;
Belaz, Katia Roberta A.
;
Tata, Alessandra
;
Santos, Vanessa Goncalves
;
da Fonseca Junior, Aldcejam Martins
;
dos Santos, Erika Cristina
;
Eberlin, Marcos Nogueira
;
Milazzotto, Marcella Pecora
Número total de Autores: 9
|
| Tipo de documento: | Artigo Científico |
| Fonte: | ZYGOTE; v. 27, n. 6, p. 10-pg., 2019-12-01. |
| Resumo | |
Human embryo studies have proposed the use of additional morphological evaluations related to the moment of the first cell divisions as relevant to embryo viability. Nevertheless, there are still not enough data available related to morphokinetic analysis and its relationship with lipid composition in embryos. Therefore, the aim of this study was to address the lipid profile of bovine embryos with different developmental kinetics: fast (four or more cells) and slow (two or three cells) at 40 h post-insemination (hpi), at three time points of in vitro culture (40, 112 and 186 hpi) and compare these to profiles of in vivo embryos. The lipid profiles of embryos were analyzed by matrix-assisted laser desorption ionization mass spectrometry, which mainly detected pools of membrane lipids such as phosphatidylcholine and sphingomyelin. In addition to their structural function, these lipid classes have an important role in cell signalling, particularly regarding events such as stress and pregnancy. Different patterns of lipids in the fast and slow groups were revealed in all the analyzed stages. Also, differences between in vitro embryos were more pronounced at 112 hpi, a critical moment due to embryonic genome activation. At the blastocyst stage, in vitro-produced embryos, despite the kinetics, had a closer lipid profile when compared with in vivo blastocysts. In conclusion, the kinetics of development had a greater effect on the membrane lipid profiles throughout the embryo culture, especially at the 8-16-cell stage. The in vitro environment affects lipid composition and may compromise cell signalling and function in blastocysts. (AU) | |
| Processo FAPESP: | 12/07206-0 - Busca de uma Assinatura Molecular da Receptividade em Bovinos por Espectrometria de Massas |
| Beneficiário: | Katia Roberta Anacleto Belaz |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |
| Processo FAPESP: | 12/10351-2 - Metabolômica para avaliação não invasiva de embriões bovinos produzidos in vitro |
| Beneficiário: | Marcella Pecora Milazzotto |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 11/06191-7 - Fingerprinting por MALDI-TOF e ESI-MS para identificação de bactérias em meios de cultura de embriões e sêmen visando a excelência sanitária da produção in vitro de embriões bovinos |
| Beneficiário: | Alessandra Tata |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |
| Processo FAPESP: | 13/13199-0 - Caracterização do metabolismo de lipídeos no desenvolvimento inicial de embriões bovinos produzidos in vitro |
| Beneficiário: | Kelly Annes |
| Modalidade de apoio: | Bolsas no Brasil - Mestrado |