Leishmania spp. in Didelphis albiventris and Micoureus paraguayanus (Didelphimorphia: Didelphidae) of Brazil

Autor(es)

Neto Quintal, Amanda Pifano ^[1] ; Ribeiro, Erica de Souza ^[1] ; Rodrigues, Fernado Pacheco ^[2] ; Rocha, Flavia Souza ^[3] ; Floeter-Winter, Lucile Maria ^[4] ; Nunes, Caris Maroni ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista, BR-16050680 Sao Paulo - Brazil

^[2] Univ Brasilia, Inst Biol Sci, Dept Genet & Morphol, BR-70910900 Brasilia, DF - Brazil

^[3] Univ Fed Rural Rio de Janeiro, Inst Forests, Dept Environm Sci, BR-23890000 Seropedica, RJ - Brazil

^[4] Univ Sao Paulo, Fac Biol, Dept Physiol, BR-05508090 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Veterinary Parasitology; v. 176, n. 2-3, p. 112-119, MAR 10 2011.
• Citações Web of Science: 5

Resumo

Leishmaniasis is kept in nature by the participation of several animal species. This study evaluated the presence of Leishmania spp. in skin samples of free-ranging marsupials Micoureus paraguayanus (n = 95) and Didelphis albiventris (n = 191), captured in Morro do Diabo State Park and in sections of its surrounding forest, in the region of Pontal do Paranapanema, Sao Paulo State, Brazil. The samples were tested for the presence of kDNA of Leishmania spp. by polymerase chain reaction (PCR) and by real time PCR (qPCR). All samples from D. albiventris tested by PCR were negative for the presence of kDNA of Leishmania spp. However, when tested by qPCR, the positivity was 1.6%. A positivity of 7.4% by PCR and 11.6% by qPCR was observed for M. paraguayanus. Sixty-four per cent (9/14) of positive animals were limited to the same forest fragment. Presence of Leishmania (Leishmania) amazonensis and Leishmania (Viannia) braziliensis was detected in M. paraguayanus samples. While D. albiventris is the most studied marsupial species due to its urban habits, other marsupial species such as M. paraguayanus can be potential reservoirs of Leishmania spp. and should also be studied. (C) 2010 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 08/07216-0 - Avaliação dos marsupiais cuíca e gambá-de-orelha-branca como reservatórios de leishmaniose
• Beneficiário: Amanda Pifano Neto Quintal
• Modalidade de apoio: Bolsas no Brasil - Mestrado