Preservation methods of fungi in 35 years old stock culture storages: A comparative study

Autor(es)

Freitas, Roseli Santos ^[1] ; Dantas, Katia Cristina ^[1] ; Pereira, Cristiane Neves ^[1] ; Levi, Jose Eduardo ^[2] ; Costa Martins, Jose Eduardo ^[1]

Afiliação do(s) autor(es)

^[1] Inst Med Trop Sao Paulo, Lab Micol Med, Sao Paulo - Brazil

^[2] Inst Med Trop Sao Paulo, Virol Lab, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: African Journal of Microbiology Research; v. 5, n. 5, p. 555-561, MAR 4 2011.
• Citações Web of Science: 1

Resumo

The maintenance of fungi isolates and the development of methodologies were available from their phenotypic and genotypic characteristics. Alterations of Histoplasma capsulatum and Candida spp. Strains preserved by continuous subculturing on fungal collection and by lyophilization for 35 years were evaluated. Candida isolates maintained by lyophilized methodology were viable and preserved by the typical characteristics of each species. Only one lyophilized isolate from H. capsulatum was viable and demonstrated typical morphology. Both genus, which were preserved by continuous subculturing, revealed morphologic alterations and lost their sporulation capacity. The DNA from these isolates was sequenced (conserved 28S rDNA) in order to confirm their identity. Random amplified polymorphism DNA (RAPD)-based comparative analysis of the two preservation methods revealed alterations in the band profiles in 28 and 33% in Candida spp. and Histoplasma capsulatum strains respectively. The RAPD-based results confirm that the subculturing method alters phenotypic and genotypic characteristics by deleting or inserting nucleotides. Otherwise, the lyophilization was effective to yeasts, but it was not effective for dimorphic fungi. (AU)

• Processo FAPESP: 02/12903-0 - Análise da variação gênica de amostras fúngicas mantidas durante 30 anos na Micoteca do IMTSP
• Beneficiário: José Eduardo Costa Martins
• Modalidade de apoio: Auxílio à Pesquisa - Regular