| Texto completo | |
| Autor(es): |
Lamarque, Giuliana de Campos Chaves
;
Leme, Roberta Duarte
;
Almeida Junior, Luciano Aparecido de
;
Bombonato-Prado, Karina Fittipaldi
;
Segato, Raquel Assed Bezerra
;
George, Anne
;
Paula-Silva, Francisco Wanderley Garcia
Número total de Autores: 7
|
| Tipo de documento: | Artigo Científico |
| Fonte: | BRAZILIAN ORAL RESEARCH; v. 39, p. 11-pg., 2025-01-01. |
| Resumo | |
Tumor necrosis factor-alpha (TNF-alpha) is a cytokine involved in the immune-inflammatory response. It can induce an odontoblastic phenotype and enhance biomineralization in dental pulp mesenchymal stem cells but does not have the same effect on osteoblasts. The reasons for this differential response, despite the shared lineage of these cell types, are not yet clear. This study examined the effects of TNF-alpha on immortalized mouse dental pulp stem cells (OD-21) and pre-osteoblastic cells (MC3T3-E1). Cells were treated with recombinant TNF-alpha at concentrations of 1, 10, and 100 ng/mL. Cell viability, proliferation, and migration were assessed using the MTT, CyQUANT, and wound healing assays, respectively. Gene expression was assessed via real-time RT-PCR, and biomineralization was evaluated using alizarin red staining. Statistical analysis was conducted using one-way ANOVA followed by Tukey's post-hoc test (alpha = 0.05). TNF-alpha did not affect cell viability at any concentration (p > 0.05). Proliferation and migration increased after 12 h, with near-complete wound closure by 24 h. TNF-alpha promoted proliferation and migration in both cell types. OD-21 cells exhibited high levels of Tnfr1 and Runx2 expression and showed biomineralization. In contrast, MC3T3-E1 cells showed high Tnfr2 levels, suppressed Runx2, and inhibited biomineralization. These results highlight how TNF-alpha influences different cell types from the same lineage in distinct ways. (AU) | |
| Processo FAPESP: | 19/00204-1 - Papel do fator de necrose tumoral-alfa na inflamação e no reparo pulpar e periapical |
| Beneficiário: | Francisco Wanderley Garcia de Paula e Silva |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 20/06607-8 - Papel dos receptores TNFR1 e TNFR2 na diferenciação de células da polpa dentária estimuladas com o fator de necrose tumoral-alfa |
| Beneficiário: | Roberta Duarte Leme |
| Modalidade de apoio: | Bolsas no Brasil - Iniciação Científica |