| Texto completo | |
| Autor(es): |
Da Silva, Caroline Caetano
;
Kollar, Timea
;
Csenki-Bakos, Zsolt
;
Fernandes, Bianca H. V.
;
Horvath, Akos
;
Carvalho, Luciani R.
Número total de Autores: 6
|
| Tipo de documento: | Artigo Científico |
| Fonte: | ZEBRAFISH; v. 16, n. 3, p. 3-pg., 2019-06-01. |
| Resumo | |
We developed an easy, efficient, and cheap protocol for zebrafish sperm cryopreservation carried out on dry ice (20min) using simple composition solution (200mM glucose, 40mM KCl, 30mM Tris, pH=8.0). The average efficiency of the present cryopreserve method was between 10% and 20% (expressed as fertilization rate). The experiments were conducted and repeated at two different locations, in different countries, yielding very similar results, showing the reproducibility and applicability of the method. (AU) | |
| Processo FAPESP: | 15/26563-7 - Diagnóstico genético molecular em pacientes com distúrbios do desenvolvimento hipofisário e gonadal e a utilização de modelos in vitro e in vivo para avaliar o efeito funcional das variantes alélicas identificadas por sequenciamento de larga escala |
| Beneficiário: | Luciani Renata Silveira de Carvalho |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |