| Texto completo | |
| Autor(es): |
Machado, Eduarda Q.
;
Salles, Barbara B. E.
;
Hantao, Leandro W.
;
de Oliveira, Cleverson J. F.
;
Arruda, Marco A. Z.
Número total de Autores: 5
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Talanta; v. 298, p. 9-pg., 2026-10-19. |
| Resumo | |
This manuscript presents a novel two-step separation method for the fractionation and speciation of nickel (Ni) and vanadium (V) metalloporphyrins in light, medium, and heavy crude oil samples using a simple paper-based chromatographic platform (5 x 5 cm). Initially, the SARA (Saturates, Aromatics, Resins, and Asphaltenes) method was employed to separate crude oil into asphaltene and maltene fractions. The asphaltene fraction underwent direct speciation, while the maltene fraction was subjected to a sequential two-step separationfractionation followed by speciation, on the same paper-based plate. Only 1 mu L of each crude oil sample and 4 mL of solvent (hexane, toluene, dichloromethane, methanol, or their mixtures) were required for the separation, which was completed in approximately 5 min. A solvent gradient elution system was used, with solvent polarity increasing incrementally every 1 cm along the plate. After separation, the analyte-containing spots were analyzed by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) to detect Ni and V associated with metalloporphyrins. Among the detected compounds, octaethylporphyrin-Ni and oxygenated-V porphyrins were identified in the more polar fractions. Their identities were confirmed by comparison with a porphyrin standard and further validated using high-resolution mass spectrometry (HRMS). This cost-effective and environmentally friendly method offers a viable alternative to conventional non-paper-based chromatographic techniques. Additionally, it facilitates the direct identification of metalloporphyrins in complex crude oil matrices, including asphaltenes, with minimal sample preparation and solvent consumption. (AU) | |
| Processo FAPESP: | 19/24445-8 - EMU concedido no processo 2018/25207-0: espectrômetro de massas com plasma indutivamente acoplado |
| Beneficiário: | Marco Aurelio Zezzi Arruda |
| Modalidade de apoio: | Auxílio à Pesquisa - Programa Equipamentos Multiusuários |
| Processo FAPESP: | 14/50867-3 - INCT 2014: Instituto Nacional de Ciência e Tecnologia de Bioanalítica |
| Beneficiário: | Marco Aurelio Zezzi Arruda |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 22/07406-1 - Emprego de ferramentas analíticas avançadas para avaliação do potencial de obtenção de bio-óleo a partir de bagaço de cana e estudo do melhoramento catalítico com vista a aplicação industrial |
| Beneficiário: | Leandro Wang Hantao |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 18/25207-0 - Nanometalômica aplicada ao cultivo da soja para estudar os efeitos das nanopartículas relacionadas a metal |
| Beneficiário: | Marco Aurelio Zezzi Arruda |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 20/08543-7 - Um kit de ferramentas especiômicas para investigar alterações mediadas por ácidos graxos na especiação plasmática de zinco e seus efeitos fisiológicos |
| Beneficiário: | Marco Aurelio Zezzi Arruda |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |