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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Grape juice concentrate prevents oxidative DNA damage in peripheral blood cells of rats subjected to a high-cholesterol diet

Texto completo
Autor(es):
Aguiar, Jr., Odair [1] ; Boiago Golluecke, Andrea Pittelli [2, 3] ; de Moraes, Barbara Bueno [1] ; Pasquini, Gabriela [1] ; Catharino, Rodrigo Ramos [4] ; Riccio, Maria Francesca [4] ; Miki Ihara, Silvia Saiuli [5] ; Ribeiro, Daniel Araki [1, 5]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Univ Fed Sao Paulo UNIFESP, Dept Biociencias, BR-11060001 Santos, SP - Brazil
[2] Univ Catolica Santos, HEXALAB, Santos - Brazil
[3] Univ Catolica Santos, Dept Nutr, Santos - Brazil
[4] Univ Estadual Campinas, Sch Med Sci, Dept Clin Pathol, Campinas, SP - Brazil
[5] Univ Fed Sao Paulo, Dept Patol, Sao Paulo - Brazil
Número total de Afiliações: 5
Tipo de documento: Artigo Científico
Fonte: BRITISH JOURNAL OF NUTRITION; v. 105, n. 5, p. 694-702, MAR 2011.
Citações Web of Science: 22
Resumo

The goal of the present study was to investigate whether subchronic treatment with grape juice concentrate is able to protect liver and peripheral blood cells against cholesterol-induced injury in rats. The effects of the grape juice concentrate treatment on histopathological changes, immunohistochemistry for cyclo-oxygenase-2 (COX-2), and basal and oxidative DNA damage induced by H(2)O(2) using a single-cell gel (comet) assay were evaluated. Male Wistar rats (n 18) were divided into three groups: group 1 - negative control; group 2 - cholesterol at 1% (w/w) in their diet, treated for 5 weeks; group 3 - cholesterol at 1% in their chow, treated for 5 weeks, and grape juice concentrate at 222 mg/d in their drinking-water in the final week only. The results indicated that the treatment with grape juice concentrate did not show remarkable differences regarding liver tissue in group 3 compared with group 2. However, grape juice concentrate was able to decrease oxidative DNA damage induced by H(2)O(2) in peripheral blood cells, as depicted by the tail moment results. COX-2 expression in the liver did not show statistically significant differences (P>0.05) between groups. Taken together, the present results suggest that the administration of subchronic grape juice concentrate prevents oxidative DNA damage in peripheral blood cells. (AU)

Processo FAPESP: 07/01228-4 - Carcinogênese bucal quimicamente induzida pela 4-nitroquinolina 1-óxido em ratos: possíveis biomarcadores envolvidos em sua patogênese
Beneficiário:Daniel Araki Ribeiro
Linha de fomento: Auxílio à Pesquisa - Apoio a Jovens Pesquisadores