Grape juice concentrate prevents oxidative DNA damage in peripheral blood cells of rats subjected to a high-cholesterol diet

Author(s)

Aguiar, Jr., Odair ^[1] ; Boiago Golluecke, Andrea Pittelli ^{[2, 3]} ; de Moraes, Barbara Bueno ^[1] ; Pasquini, Gabriela ^[1] ; Catharino, Rodrigo Ramos ^[4] ; Riccio, Maria Francesca ^[4] ; Miki Ihara, Silvia Saiuli ^[5] ; Ribeiro, Daniel Araki ^{[1, 5]}

Affiliation

^[1] Univ Fed Sao Paulo UNIFESP, Dept Biociencias, BR-11060001 Santos, SP - Brazil

^[2] Univ Catolica Santos, HEXALAB, Santos - Brazil

^[3] Univ Catolica Santos, Dept Nutr, Santos - Brazil

^[4] Univ Estadual Campinas, Sch Med Sci, Dept Clin Pathol, Campinas, SP - Brazil

^[5] Univ Fed Sao Paulo, Dept Patol, Sao Paulo - Brazil

• Document type: Journal article
• Source: BRITISH JOURNAL OF NUTRITION; v. 105, n. 5, p. 694-702, MAR 2011.
• Web of Science Citations: 22

Abstract

The goal of the present study was to investigate whether subchronic treatment with grape juice concentrate is able to protect liver and peripheral blood cells against cholesterol-induced injury in rats. The effects of the grape juice concentrate treatment on histopathological changes, immunohistochemistry for cyclo-oxygenase-2 (COX-2), and basal and oxidative DNA damage induced by H(2)O(2) using a single-cell gel (comet) assay were evaluated. Male Wistar rats (n 18) were divided into three groups: group 1 - negative control; group 2 - cholesterol at 1% (w/w) in their diet, treated for 5 weeks; group 3 - cholesterol at 1% in their chow, treated for 5 weeks, and grape juice concentrate at 222 mg/d in their drinking-water in the final week only. The results indicated that the treatment with grape juice concentrate did not show remarkable differences regarding liver tissue in group 3 compared with group 2. However, grape juice concentrate was able to decrease oxidative DNA damage induced by H(2)O(2) in peripheral blood cells, as depicted by the tail moment results. COX-2 expression in the liver did not show statistically significant differences (P>0.05) between groups. Taken together, the present results suggest that the administration of subchronic grape juice concentrate prevents oxidative DNA damage in peripheral blood cells. (AU)

• FAPESP's process: 07/01228-4 - Medium-term oral carcinogenesis assay induced by 4-nitroquinoline 1-oxide in rats: putative biomarkers involved into its pathogenesis
• Grantee: Daniel Araki Ribeiro
• Support Opportunities: Research Grants - Young Investigators Grants