Influence of Suture on Peripheral Nerve Regeneration and Collagen Production at the Site of Neurorrhaphy: An Experimental Study

Autor(es)

Martins, Roberto Sergio ^{[1, 2]} ; Teodoro, Walcy Rosolio ^[3] ; Simplicio, Hougelle ^[4] ; Capellozi, Vera Luiza ^[5] ; Siqueira, Mario Gilberto ^[6] ; Yoshinari, Natalino Hajime ^[3] ; Pereira Plese, Jose Pindaro ^[4] ; Teixeira, Manoel Jacobsen ^[4]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Med, Dept Neurosurg, Peripheral Nerve Surg Unit, Sao Paulo - Brazil

^[2] Hosp Servidor Publ Estado, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Sch Med, Div Rheumatol, Sao Paulo - Brazil

^[4] Univ Sao Paulo, Sch Med, Dept Neurosurg, Sao Paulo - Brazil

^[5] Univ Sao Paulo, Sch Med, Dept Pathol, Sao Paulo - Brazil

^[6] Univ Sao Paulo, Sch Med, Peripheral Nerve Surg Unit, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: NEUROSURGERY; v. 68, n. 3, p. 765-772, MAR 2011.
• Citações Web of Science: 12

Resumo

BACKGROUND: Restoration of nerve continuity and effective maintenance of coaptation are considered fundamental principles of end-to-end peripheral nerve repair. OBJECTIVE: To evaluate the influence of the number of stitches on axonal regeneration and collagen production after neurorrhaphy. METHODS: Thirty male Wistar rats were equally divided into 3 groups and were all operated on with the right sciatic nerve exposed. In 2 groups, the nerve was sectioned and repaired by means of 3 (group B) or 6 (group C) epineurium sutures with 100 monofilament nylon. One group (group A) was used as a control. Each animal from groups B and C underwent electrophysiological evaluation with motor action potential recordings before nerve section and again at an 8-week interval after neurorrhaphy. Nerve biopsy specimens were used for histomorphometric assessment of axonal regeneration and quantification of collagen at the repair site. RESULTS: Animals from group C had significantly lower motor action potential conduction velocities compared with control animals (P = .02), and no significant difference was seen between groups B and C. Parameters obtained from morphometric evaluation were not significantly different between these 2 groups. Type I collagen and III collagen in the epineurium were significantly higher in group C than in either the control group (P = .001 and P = .003) or group B (P = .01 and P = .02). No differences were identified for collagen I and III in the endoneurium. CONCLUSION: Using 6 sutures for nerve repair is associated with worse electrophysiological outcomes and higher amounts of type I and III collagen in the epineurium compared with control. Neurorraphy with 6 stitches is also related to a significant increase in epineurium collagen I and III compared with 3-stitch neurorraphy. (AU)

• Processo FAPESP: 05/52138-0 - Influencia do numero de pontos na regeneracao axonal e producao de matriz extracelular na sutura epineural termino-terminal do nervo ciatico do rato.
• Beneficiário: José Pindaro Pereira Plese
• Modalidade de apoio: Auxílio à Pesquisa - Regular