Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Influence of Suture on Peripheral Nerve Regeneration and Collagen Production at the Site of Neurorrhaphy: An Experimental Study

Full text
Author(s):
Martins, Roberto Sergio [1, 2] ; Teodoro, Walcy Rosolio [3] ; Simplicio, Hougelle [4] ; Capellozi, Vera Luiza [5] ; Siqueira, Mario Gilberto [6] ; Yoshinari, Natalino Hajime [3] ; Pereira Plese, Jose Pindaro [4] ; Teixeira, Manoel Jacobsen [4]
Total Authors: 8
Affiliation:
[1] Univ Sao Paulo, Sch Med, Dept Neurosurg, Peripheral Nerve Surg Unit, Sao Paulo - Brazil
[2] Hosp Servidor Publ Estado, Sao Paulo - Brazil
[3] Univ Sao Paulo, Sch Med, Div Rheumatol, Sao Paulo - Brazil
[4] Univ Sao Paulo, Sch Med, Dept Neurosurg, Sao Paulo - Brazil
[5] Univ Sao Paulo, Sch Med, Dept Pathol, Sao Paulo - Brazil
[6] Univ Sao Paulo, Sch Med, Peripheral Nerve Surg Unit, Sao Paulo - Brazil
Total Affiliations: 6
Document type: Journal article
Source: NEUROSURGERY; v. 68, n. 3, p. 765-772, MAR 2011.
Web of Science Citations: 12
Abstract

BACKGROUND: Restoration of nerve continuity and effective maintenance of coaptation are considered fundamental principles of end-to-end peripheral nerve repair. OBJECTIVE: To evaluate the influence of the number of stitches on axonal regeneration and collagen production after neurorrhaphy. METHODS: Thirty male Wistar rats were equally divided into 3 groups and were all operated on with the right sciatic nerve exposed. In 2 groups, the nerve was sectioned and repaired by means of 3 (group B) or 6 (group C) epineurium sutures with 100 monofilament nylon. One group (group A) was used as a control. Each animal from groups B and C underwent electrophysiological evaluation with motor action potential recordings before nerve section and again at an 8-week interval after neurorrhaphy. Nerve biopsy specimens were used for histomorphometric assessment of axonal regeneration and quantification of collagen at the repair site. RESULTS: Animals from group C had significantly lower motor action potential conduction velocities compared with control animals (P = .02), and no significant difference was seen between groups B and C. Parameters obtained from morphometric evaluation were not significantly different between these 2 groups. Type I collagen and III collagen in the epineurium were significantly higher in group C than in either the control group (P = .001 and P = .003) or group B (P = .01 and P = .02). No differences were identified for collagen I and III in the endoneurium. CONCLUSION: Using 6 sutures for nerve repair is associated with worse electrophysiological outcomes and higher amounts of type I and III collagen in the epineurium compared with control. Neurorraphy with 6 stitches is also related to a significant increase in epineurium collagen I and III compared with 3-stitch neurorraphy. (AU)