A single multiplex PCR and SNaPshot minisequencing reaction of 42 SNPs to classify admixture populations into mitochondrial DNA haplogroups

Autor(es)

Paneto, Greiciane G. ^[1] ; Koehnemann, Stephan ^[2] ; Martins, Joyce A. ^[1] ; Cicarelli, Regina M. B. ^[1] ; Pfeiffer, Heidi ^[2]

Afiliação do(s) autor(es)

^[1] Unesp Univ Estadual Paulista, Fac Ciencias Farmaceut, Lab Invest Paternidade, BR-14801902 Araraquara, SP - Brazil

^[2] Univ Munster, Inst Legal Med, D-48149 Munster - Germany

• Tipo de documento: Artigo Científico
• Fonte: MITOCHONDRION; v. 11, n. 2, p. 296-302, MAR 2011.
• Citações Web of Science: 21

Resumo

SNaPshot minisequencing reaction is in increasing use because of its fast detection of many polymorphisms in a single assay. In this work we described a highly sensitive single nucleotide polymorphisms (SNPs) typing method with detection of 42 mitochondrial DNA (mtDNA) SNPs in a single PCR and SNaPshot multiplex reaction, in order to allow haplogroup classification in Latin American admixture population. We validated the panel typing 160 Brazilian individuals. Complete SNP profiles were obtained from 10 pg of total DNA. We conclude that it is possible to build and genotype more than forty mtDNA SNPs in a single multiplex PCR and SNaPshot reaction, with sensitivity and reliability, resolving haplogroup classification in admixture populations. (C) 2010 Elsevier B.V. and Mitochondria Research Society. All rights reserved. (AU)

• Processo FAPESP: 05/03984-5 - Utilizacao do dna mitocondrial na analise forense.
• Beneficiário: Regina Maria Barretto Cicarelli
• Modalidade de apoio: Auxílio à Pesquisa - Regular