Comparison of Endotoxin Levels in Previous Studies on Primary Endodontic Infections

Autor(es)

Martinho, Frederico C. ^[1] ; Chiesa, Wanderson M. M. ^[1] ; Zaia, Alexandre A. ^[1] ; Ferraz, Caio C. R. ^[1] ; Almeida, Jose F. A. ^[1] ; Souza-Filho, Francisco J. ^[1] ; Gomes, Brenda P. F. A. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Endodont Div, Piracicaba Dent Sch, Dept Restorat Dent, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF ENDODONTICS; v. 37, n. 2, p. 163-167, FEB 2011.
• Citações Web of Science: 22

Resumo

Background: This study was performed to determine which of the quantitative methods, namely, chromogenic endpoint, chromogenic kinetic, and turbidimetric kinetic ones, best fit for the analysis of primary endodontic infections. Methods: Twenty-one root canals with apical periodontitis were sampled with paper points. The same sample was analyzed by means of the endpoint chromogenic Limulus amebocyte lysate (LAL) assay (QCL), quantitative kinetic chromogenic LAL assay (KQCL), and kinetic turbidimetric LAL assay (Turbidimetric). Results: All three LAL methods were effective in the recovery of endotoxin from root canal infection. Regardless of the method tested, endotoxin was detected in 100% of the root canals (21/21). The KQCL assay yielded a median value of endotoxin of 7.49 EU/mL, close to and not significantly different from those for the turbidimetric test (9.19 EU/mL) (both kinetic methods) (p > 0.05). In contrast, the endpoint QCL showed a median value of 34.20 EU/mL (p < 0.05). The comparison of the three methods revealed that both turbidimetric and KQCL methods were more precise, with best reproducibility (the coefficient variation between analysis of the root canal and its duplicate was lower than 10%). The inhibition/enhancement assay indicated a good interaction between the root canal samples with the turbidimetric method. Conclusion: This study has revealed that quantitative kinetic-turbidimetric and kinetic-chromogenic LAL methods are best fitted for the analysis of endotoxins in root canal infection, both being more precise and allowing better reproducibility compared with the endpoint-QCL assay. (J Endod 2011;37:163-167) (AU)

• Processo FAPESP: 07/58518-4 - Análise da microbiota de canais radiculares de dentes humanos infectados através da cultura, clonagem e sequenciamento do gene 16S rRNA
• Beneficiário: Brenda Paula Figueiredo de Almeida Gomes
• Linha de fomento: Auxílio à Pesquisa - Regular
• Processo FAPESP: 08/57954-8 - Estudo microbiológico, quantificação de endotoxinas e investigação da atividade endotóxica de diferentes LPS bacterianos isolados de canais radiculares de dentes com necrose pulpar e presença de lesão periapiacal
• Beneficiário: Frederico Canato Martinho
• Linha de fomento: Bolsas no Brasil - Doutorado
• Processo FAPESP: 08/58299-3 - Estudo microbiológico e de endotoxinas em canais radiculares de dentes com necrose pulpar e lesão periapical e avaliação da atividade endotóxica de LPS isolados de canais radiculares infectados
• Beneficiário: Brenda Paula Figueiredo de Almeida Gomes
• Linha de fomento: Auxílio à Pesquisa - Regular