Temporal Changes in Matrix Metalloproteinases, Their Inhibitors, and Cathepsins in Mouse Pubic Symphysis During Pregnancy and Postpartum

Autor(es)

Rosa, Renata G. ^[1] ; Tarsitano, Christiane A. B. ^[2] ; Hyslop, Stephen ^[2] ; Yamada, Aureo T. ^[1] ; Toledo, Olga M. S. ^[3] ; Joazeiro, Paulo P. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas UNICAMP, Dept Histol & Embryol, Inst Biol, BR-13083970 Campinas, SP - Brazil

^[2] Univ Estadual Campinas UNICAMP, Dept Pharmacol, Fac Med Sci, BR-13083970 Campinas, SP - Brazil

^[3] Fac Med ABC, Dept Morfol, Santo Andre, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: REPRODUCTIVE SCIENCES; v. 18, n. 10, p. 963-977, 2011.
• Citações Web of Science: 4

Resumo

Remodeling and relaxation of the mouse pubic symphysis (PS) are central events in parturition. The involvement of endogenous proteins such as matrix metalloproteinases (MMPs), tissue inhibitors of matrix metalloproteinases (TIMPs), and cathepsins in these phenomena remains unclear. In this work, we used a combination of immunolocalization, protein expression/activity, and relative messenger RNA (mRNA) expression to examine the changes in selected MMPs (-2, -9, and -8), TIMPs (-1 and -2), and cathepsins (B and K) during pregnancy and postpartum in mice. Immunohistochemistry revealed the presence of all of these proteins in the cytoplasm of chondrocytes, fibrochondrocytes, and fibroblast-like cells in the interpubic tissues. Zymography showed increases in the active forms of MMP-2 and -9 primarily on days 15 to 19 of pregnancy. Western blotting showed enhanced expression of MMP-8 on days 12 to 15 of pregnancy, with no changes in cathepsins B and K. Matrix metalloproteinases 2, TIMP-1 and -2, and cathepsin B had significant relative gene expression throughout pregnancy. These findings indicate that during pregnancy and postpartum there are variations in the expression and activity of proteins that may have an important role in remodeling the pubic symphysis during these events. (AU)

• Processo FAPESP: 05/51844-8 - Detecção de metaloproteinases da família das gelatinases e de seus inibidores no ligamento interpúbico do camundongo, durante a prenhez
• Beneficiário: Renata Giardini Rosa
• Modalidade de apoio: Bolsas no Brasil - Mestrado