| Texto completo | |
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De Bem, Tiago H. C.
[1]
;
Chiaratti, Marcos R.
[1]
;
Rochetti, Raquel
[1]
;
Bressan, Fabiana F.
[1]
;
Sangalli, Juliano R.
[1]
;
Miranda, Moyses S.
[2, 1]
;
Pires, Pedro R. L.
[1]
;
Schwartz, Katia R. L.
[1]
;
Sampaio, Rafael V.
[2, 1]
;
Fantinato-Neto, Paulo
[1]
;
Pimentel, Jose R. V.
[1]
;
Perecin, Felipe
[1]
;
Smith, Lawrence C.
[3, 1]
;
Meirelles, Flavio V.
[1]
;
Adona, Paulo R.
[4, 1]
;
Leal, Claudia L. V.
[1]
Número total de Autores: 16
|
| Afiliação do(s) autor(es): | [1] Univ Sao Paulo, Fac Zootecnia & Engn Alimentos, Dept Ciencias Basicas, BR-13635900 Pirassununga, SP - Brazil
[2] Fed Univ Para, Inst Ciencias Biol, Dept Biol, BR-66059 Belem, Para - Brazil
[3] Univ Montreal, Fac Med Vet, Ctr Rech Reprod Anim, St Hyacinthe, PQ J2S 7C6 - Canada
[4] Univ Norte Parana, Londrina, PR - Brazil
Número total de Afiliações: 4
|
| Tipo de documento: | Artigo Científico |
| Fonte: | CELLULAR REPROGRAMMING; v. 13, n. 5, p. 419-429, 2011. |
| Citações Web of Science: | 13 |
| Resumo | |
Somatic cell nuclear transfer (SCNT) has had an enormous impact on our understanding of biology and remains a unique tool for multiplying valuable laboratory and domestic animals. However, the complexity of the procedure and its poor efficiency are factors that limit a wider application of SCNT. In this context, oocyte meiotic arrest is an important option to make SCNT more flexible and increase the number of cloned embryos produced. Herein, we show that the use of butyrolactone I in association with brain-derived neurotrophic factor (BDNF) to arrest the meiotic division for 24 h prior to in vitro maturation provides bovine (Bos indicus) oocytes capable of supporting development of blastocysts and full-term cloned calves at least as efficiently as nonarrested oocytes. Furthermore, the procedure resulted in cloned blastocysts with an 1.5- and twofold increase of POU5F1 and IFNT2 expression, respectively, which are well-known markers of embryonic viability. Mitochondrial DNA (mtDNA) copy number was diminished by prematuration in immature oocytes (718,585 +/- 34,775 vs. 595,579 +/- 31,922, respectively, control and treated groups) but was unchanged in mature oocytes (522,179 +/- 45,617 vs. 498,771 +/- 33,231) and blastocysts (816,627 +/- 40,235 vs. 765,332 +/- 51,104). To our knowledge, this is the first report of cloned offspring born to prematured oocytes, indicating that meiotic arrest could have significant implications for laboratories working with SCNT and in vitro embryo production. (AU) | |
| Processo FAPESP: | 06/58536-0 - Efeito da pre maturacao sobre o desenvolvimento embrionario de oocitos submetidos a ativacao partenogenetica e transferencia de nucleo. |
| Beneficiário: | Tiago Henrique Camara De Bem |
| Modalidade de apoio: | Bolsas no Brasil - Mestrado |
| Processo FAPESP: | 05/59694-5 - Avaliacao de rnam diferencialmente transcritos em ovocitos bovinos maturados in vivo e in vitro para identificacao de possiveis marcadores de competencia ovocitaria. |
| Beneficiário: | Cláudia Lima Verde Leal |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |