Effects of 7-Epiclusianone on Streptococcus mutans and Caries Development in Rats

Autor(es)

Branco-de-Almeida, Luciana Salles ^[1] ; Murata, Ramiro Mendonca ^[1] ; Franco, Eliane Melo ^[1] ; dos Santos, Marcelo Henrique ; de Alencar, Severino Matias ^[2] ; Koo, Hyun ^{[3, 4]} ; Rosalen, Pedro Luiz ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Piracicaba Dent Sch, Dept Physiol Sci, BR-13414903 Piracicaba, SP - Brazil

^[2] Univ Sao Paulo, Dept Agrifood Ind Food & Nutr, Escola Super Agr Luiz de Queiroz, Piracicaba, SP - Brazil

^[3] Univ Rochester, Med Ctr, Ctr Oral Biol, Rochester, NY 14642 - USA

^[4] Univ Rochester, Med Ctr, Eastman Dept Dent, Rochester, NY 14642 - USA

• Tipo de documento: Artigo Científico
• Fonte: Planta Medica; v. 77, n. 1, p. 40-45, JAN 2011.
• Citações Web of Science: 17

Resumo

The aim of this study was to evaluate the effects of 7-epiclusianone (7-epi) on specific virulence attributes of Streptococcus mutans in vitro and on development of dental caries in vivo. 7-Epi was obtained and purified from fruits of Rheedia brasiliensis. We investigated its influence on surface-adsorbed glucosyltransferase (Gtf) B activity, acid production, and viability of S. mutans in biofilms, as well as on caries development using a rodent model. 7-Epi (100 mu g/mL) significantly reduced the activity of surface-adsorbed GtfB (up to 48.0 +/- 1.8 of inhibition at 100 mu g/mL) and glyco-lytic pH-drop by S. mutans in biofilms (125 and 250 mu g/mL) (vs. vehicle control, p < 0.05). In contrast, the test compound did not significantly affect the bacterial viability when compared to vehicle control (15% ethanol, p > 0.05). Wistar rats treated topically with 7-epi (twice daily, 60-s exposure) showed significantly smaller number of and less severe smooth-and sulcal-surface carious lesions (p < 0.05), without reducing the S. mutans viable population from the animals' dental biofilms. In conclusion, the natural compound 7-epiclusianone may be a potentially novel pharmacological agent to prevent and control dental caries disease. (AU)

• Processo FAPESP: 06/56379-4 - Potencial antimicrobiano de um novo composto poliprenilado extraido da rheedia brasiliensis e sua influencia na expressao proteica de biofilmes de streptococcus mutans.
• Beneficiário: Maria Cristina Volpato
• Modalidade de apoio: Auxílio à Pesquisa - Regular