Vibrio cholerae O1 detection in estuarine and coastal zooplankton

Autor(es)

Martinelli Filho, Jose E. ^{[1, 2]} ; Lopes, Rubens M. ^[1] ; Rivera, Irma N. G. ^[3] ; Colwell, Rita R. ^{[4, 5]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Oceanog Inst, Biol Oceanog Dept, BR-05508120 Sao Paulo - Brazil

^[2] Fed Univ Para, Fac Biol Sci, BR-68372040 Altamira, PA - Brazil

^[3] Univ Sao Paulo, Inst Biomed Sci, Dept Microbiol, BR-05508900 Sao Paulo - Brazil

^[4] Univ Maryland, Inst Biotechnol, Ctr Marine Biotechnol, Baltimore, MD 21202 - USA

^[5] Univ Maryland, Ctr Bioinformat & Computat Biol, College Pk, MD 20742 - USA

• Tipo de documento: Artigo Científico
• Fonte: Journal of Plankton Research; v. 33, n. 1, p. 51-62, JAN 2011.
• Citações Web of Science: 17

Resumo

Vibrio cholerae is an autochthonous marine bacterium, and its association with diverse planktonic crustaceans has been extensively investigated; however, the presence of V. cholerae on individuals of most phyla of planktonic animals is still incompletely understood. The objective of this study was to analyze the distribution of V. cholerae serogroup O1 associated with specific zooplankton taxa in an estuary and the adjacent continental shelf of the southeastern Brazilian coast. The occurrence of the bacterium was assessed in zooplankton samples, specifically on the most abundant taxa, using direct fluorescence assay (DFA) and direct viable count-direct fluorescence assay (DVC-DFA) methods. Vibrio cholerae O1 was detected in 88% of samples collected from the Santos-Bertioga estuary and in 67% of samples from the shelf. The salinity of the estuarine water ranged from 21.8 to 34.6, significantly lower than the shelf water which was 32.1-36.1. Salinity was the only environmental variable measured that displayed a significant correlation with the presence of V. cholerae (P < 0.05). Vibrio cholerae O1 was detected in chaetognaths, pluteus larvae of echinoderms and planktonic fish eggs (Engraulidae), all new sites for this bacterium. (AU)

• Processo FAPESP: 03/09932-1 - A influência do complexo estuarino da Baixada Santista sobre o ecossistema da plataforma adjacente (ECOSAN)
• Beneficiário: Ana Maria Setubal Pires-Vanin
• Linha de fomento: Auxílio à Pesquisa - Programa PRONEX - Temático