Investigating Giant (Gt) Repression in the Formation of Partially Overlapping Pair-Rule Stripes

Autor(es)

Ribeiro, Thiago Case ^[1] ; Ventrice, Glauber ^[1] ; Machado-Lima, Ariane ^[2] ; Andrioli, Luiz Paulo ^{[2, 1]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Biociencias, Dept Genet & Biol Evolut, BR-03828000 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Escola Artes Ciencias & Humanidades, BR-03828000 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: DEVELOPMENTAL DYNAMICS; v. 239, n. 11, p. 2989-2999, NOV 2010.
• Citações Web of Science: 3

Resumo

Drosophila pair-rule genes are expressed in striped patterns with a precise order of overlap between stripes of different genes. We investigated the role of Giant (Gt) in the regulation of even-skipped, hairy, runt, and fushi tarazu stripes formed in the vicinity of Gt expression domains. In gt null embryos, specific stripes of eve, h, run, and ftz are disrupted. With an ectopic expression system, we verified that stripes affected in the mutant are also repressed. Simultaneously hybridizing gt misxpressing embryos with two pair-rule gene probes, we were able to distinguish differences in the repression of pairs of stripes that overlap extensively. Together, our results showed Gt repression roles in the regulation of two groups of partially overlapping stripes and that Gt morphogen activity is part of the mechanism responsible for the differential positioning of these stripes borders. We discuss the possibility that other factors regulate Gt stripe targets as well. Developmental Dynamics 239:2989-2999, 2010. (C) 2010 Wiley-Liss, Inc. (AU)

• Processo FAPESP: 05/58898-6 - Repressão transcricional na formação do padrão estriado pair-rule de Drosophila melanogaster
• Beneficiário: Glauber de Morais Ventrice
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 07/55034-6 - Investigação do gene Forkhead CG9571 na cascata de segmentação de Drosophila melanogaster
• Beneficiário: Thiago Case Pimenta Ribeiro
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 03/01640-1 - Regulação dos genes de segmentação expressos na cabeça no início do desenvolvimento de Drosophila melanogaster
• Beneficiário: Luiz Paulo Moura Andrioli
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 03/12147-4 - Regulação dos genes de segmentação expressos na cabeça no início do desenvolvimento de Drosophila melanogaster
• Beneficiário: Luiz Paulo Moura Andrioli
• Modalidade de apoio: Bolsas no Brasil - Jovens Pesquisadores