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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Investigating Giant (Gt) Repression in the Formation of Partially Overlapping Pair-Rule Stripes

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Author(s):
Ribeiro, Thiago Case [1] ; Ventrice, Glauber [1] ; Machado-Lima, Ariane [2] ; Andrioli, Luiz Paulo [2, 1]
Total Authors: 4
Affiliation:
[1] Univ Sao Paulo, Inst Biociencias, Dept Genet & Biol Evolut, BR-03828000 Sao Paulo - Brazil
[2] Univ Sao Paulo, Escola Artes Ciencias & Humanidades, BR-03828000 Sao Paulo - Brazil
Total Affiliations: 2
Document type: Journal article
Source: DEVELOPMENTAL DYNAMICS; v. 239, n. 11, p. 2989-2999, NOV 2010.
Web of Science Citations: 3
Abstract

Drosophila pair-rule genes are expressed in striped patterns with a precise order of overlap between stripes of different genes. We investigated the role of Giant (Gt) in the regulation of even-skipped, hairy, runt, and fushi tarazu stripes formed in the vicinity of Gt expression domains. In gt null embryos, specific stripes of eve, h, run, and ftz are disrupted. With an ectopic expression system, we verified that stripes affected in the mutant are also repressed. Simultaneously hybridizing gt misxpressing embryos with two pair-rule gene probes, we were able to distinguish differences in the repression of pairs of stripes that overlap extensively. Together, our results showed Gt repression roles in the regulation of two groups of partially overlapping stripes and that Gt morphogen activity is part of the mechanism responsible for the differential positioning of these stripes borders. We discuss the possibility that other factors regulate Gt stripe targets as well. Developmental Dynamics 239:2989-2999, 2010. (C) 2010 Wiley-Liss, Inc. (AU)