Effects of different closes and schedules of diazepam treatment on lymphocyte parameters in rats

Autor(es)

de Lima, Camila Bento ^{[1, 2]} ; Sakai, Monica ^[2] ; Latorre, Andreia Oliveira ^[2] ; de Moraes Moreau, Regina Lucia ^[1] ; Palermo-Neto, Jodo ^[2]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Pharmaceut Sci, Dept Clin & Toxicol Anal, BR-05508000 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Lab Appl Pharmacol & Toxicol, Sch Vet Med, Neuroimmunomodulat Res Grp, BR-05508000 Sao Paulo - Brazil

• Tipo de documento: Artigo de Revisão
• Fonte: International Immunopharmacology; v. 10, n. 11, p. 1335-1343, NOV 2010.
• Citações Web of Science: 8

Resumo

Benzodiazepines (BZD) are widely used for the treatment of anxiety. They enhance GABA-ergic neurotransmission through the binding on specific BDZ recognition sites, within the GABA(A) receptor-ion channel complex. However, recent studies showed that BZD also act on peripheral benzodiazepine receptor sites (PBR) or translocator protein 18 kDa (TSPO). Evidence for a direct immunomodulatory action for BZD emerged from studies that demonstrated the presence of TSPO on immune/inflammatory cells. The present study was designed to analyze the effects of diazepam on rat lymphocyte parameters, specifically on phenotype, cell proliferation and cell death. The effects of both acute and long-term (21 days) diazepam (1 and 10 mg/kg/day) administrations were evaluated. Results showed that diazepam (1 mg/kg) treatment did not change the immune parameters analyzed. However, both diazepam (10 mg/kg) acute and long-term treatments decreased the number of apoptotic cells; they also increased the percentage of T cytotoxic cells; decreased the percentage of B cells and increased the corticosterone serum levels. The induction of functional tolerance was suggested for the highest dose of diazepam (10 mg/kg), but not for the smaller dose (1 mg/kg) used, at least for diazepam effects on corticosterone serum levels. Diazepam effects were discussed as being related to the number of TSPO sites present on immune cells and/or to the increased levels of serum corticosterone observed after the treatments used. (C) 2010 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 06/58540-7 - Efeitos da administração aguda e prolongada de diazepam sobre parâmetros imunológicos e bioquímicos de ratos
• Beneficiário: Camila Bento de Lima
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 04/14128-0 - Neuroimunomodulação: efeitos do estresse e de citocinas nas relações bidirecionais entre os sistemas nervosos central e imune
• Beneficiário: João Palermo Neto
• Modalidade de apoio: Auxílio à Pesquisa - Temático