Apoptotic and developmental effects of bovine Herpesvirus type-5 infection on in vitro-produced bovine embryos

Autor(es)

Silva-Frade, C. ^{[1, 2]} ; Gameiro, R. ^[1] ; Martins, Jr., A. ^[2] ; Cardoso, T. C. ^[1]

Afiliação do(s) autor(es)

^[1] UNESP Univ Sao Paulo State, Coll Vet Med, Lab Anim Virol, BR-16050680 Sao Paulo - Brazil

^[2] UNESP Univ Sao Paulo State, Coll Vet Med, Lab Anim Reprod, BR-16050680 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Theriogenology; v. 74, n. 7, p. 1296-1303, OCT 15 2010.
• Citações Web of Science: 11

Resumo

Bovine Herpesvirus type-5 (BoHV-5), which is potentially neuropathogenic, was recently described to be related with reproductive disorders in cows. The objective was to elucidate mechanisms involved in propagation of BoHV-5 in embryonic cells. For this purpose, bovine embryos produced in vitro were assayed for apoptotic markers after experimental infection of oocytes, in vitro fertilization, and development. Host DNA fragmentation was detected with a TUNEL assay, expression of annexin-V was measured with indirect immunofluorescence, and viral DNA was detected with in situ hybridization. Infective BoHV-5 virus was recovered from embryos derived from exposed oocytes after two consecutive passages on Madin-Darby bovine kidney (MDBK) cells. The viral DNA corresponding to US9 gene, localized between nucleotides 126243 to 126493, was detected in situ and amplified. There was no significant difference between the ratio of TUNEL stained nuclei and total cells in good quality blastocysts (0.87 +/- 0.05, mean SD), but there were differences (P < 0.05) between infected (0.18 +/- 0.05) and uninfected blastocysts (0.73 +/- 0.07). The Annexin-V label was more intense in uninfected embryos (0.79 +/- 0.04; P < 0.05). The quality of infected and uninfected embryos was considered equal, with no significant effect on embryonic development. In conclusion, we inferred that BoHV-5 infected bovine oocytes, replicated, and suppressed some apoptotic pathways, without significantly affecting embryonic development. (C) 2010 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 07/57774-7 - Infeccao experimental com herpesvirus bovino tipo 5 e virus da diarreia viral bovina em gametas e embrioes bovinos produzidos in vitro.
• Beneficiário: Camila da Silva Frade
• Modalidade de apoio: Bolsas no Brasil - Mestrado