Microbiota associated with chronic osteomyelitis of the jaws

Autor(es)

Gaetti-Jardim Junior, Elerson ^{[1, 2]} ; Fardin, Angelica Cristiane ^[2] ; Gaetti-Jardim, Ellen Cristina ^[2] ; de Castro, Alvimar Lima ^[2] ; Schweitzer, Christiane Marie ^[3] ; Avila-Campos, Mario Julio ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Lab Anaerobios, Dept Microbiol, Sao Paulo - Brazil

^[2] Univ Estadual Paulista, Fac Odontol Aracatuba, Dept Patol, Aracatuba, SP - Brazil

^[3] Univ Fed ABC, Ctr Matemat Computacao & Cognicao, Santo Andre, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Journal of Microbiology; v. 41, n. 4, p. 1056-1064, OCT-DEC 2010.
• Citações Web of Science: 9

Resumo

Chronic osteomyelitis of maxilla and mandible is rare in industrialized countries and its occurrence in developing countries is associated with trauma and surgery, and its microbial etiology has not been studied thoroughly. The aim of this investigation was to evaluate the microbiota associated with osteomyelitis of mandible or maxilla from some Brazilian patients. After clinical and radiographic evaluation, samples of bone sequestra, purulent secretion, and biopsies of granulomatous tissues from twenty-two patients with chronic osteomyelitis of mandible and maxilla were cultivated and submitted for pathogen detection by using a PCR method. Each patient harbored a single lesion. Bacterial isolation was performed on fastidious anaerobe agar supplemented with hemin, menadione and horse blood for anaerobes; and on tryptic soy agar supplemented with yeast extract and horse blood for facultative bacteria and aerobes. Plates were incubated in anaerobiosis and aerobiosis, at 37ºC for 14 and 3 days, respectively. Bacteria were cultivated from twelve patient samples; and genera Actinomyces, Fusobacterium, Parvimonas, and Staphylococcus were the most frequent. By PCR, bacterial DNA was detected from sixteen patient samples. The results suggest that cases of chronic osteomyelitis of the jaws are usually mixed anaerobic infections, reinforcing the concept that osteomyelitis of the jaws are mainly related to microorganisms from the oral environment, and periapical and periodontal infections may act as predisposing factors. (AU)

• Processo FAPESP: 02/07371-0 - Avaliação dos principais fatores de risco da cárie dentária em uma população com atenção precoce
• Beneficiário: Elerson Gaetti Jardim Júnior
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 07/54851-0 - Avaliação da microbiota bucal de pacientes submetidos à radioterapia para tratamento de lesões malignas de cabeça e pescoço
• Beneficiário: Elerson Gaetti Jardim Júnior
• Modalidade de apoio: Auxílio à Pesquisa - Regular