Trypanocidal, leislimanicidal and antifungal potential from marine red alga Bostrychia tenella J. Agardli (Rhodomelaceae, Ceraniiales)

Autor(es)

de Felicio, Rafael ^[1] ; de Albuquerque, Sergio ^[2] ; Marx Young, Maria Claudia ^[3] ; Yokoya, Nair Sumie ^[4] ; Debonsi, Hosana Maria ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Quim & Fis, Fac Ciencias Farmaceut Ribeirao Preto, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Dept Anal Clin Toxicol & Biomatol, Fac Ciencias Farmaceut Ribeirao Preto, Sao Paulo - Brazil

^[3] Inst Bot Sao Paulo, Secao Fisiol & Bioquim Vegetal, Sao Paulo - Brazil

^[4] Secretaria Estado Meio Ambiente, Secao Ficol, Inst Bot, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Pharmaceutical and Biomedical Analysis; v. 52, n. 5, p. 763-769, SEP 5 2010.
• Citações Web of Science: 29

Resumo

Specimens of the red alga Bostrychia tenella J Agardh (Rhodomelaceae, Ceramiales) were collected from the Sao Paulo coast and submitted to loom temperature solvent extraction The resulting extract was fractionated by partitioning with organic solvent The n-hexane (BT-H) and dichloromethane (BT-D) fractions showed antiprotozoal potential in biological tests with Trypanosoma cruzi and Leishmania amazonensis and presented high activity in an antifungal assay with the phytopathogenic fungi Cladosporium cladosporioides and Cladosporium sphaerospermum Chromatography methods were used to generate subfractions from BT-H (H01 to H11) and from BT-D (D01 to 019) The subtractions were analyzed by gas chromatography-mass spectrometry (GC/MS). and the substances were identified by retention index (Kovats) and by comparison to databases of commercial mass spectra The volatile compounds found in marine algae were identified as fatty acids, low molecular mass hydrocarbons, esters and steroids, some of these have been previously described in the literature based on other biological activities Moreover, uncommon substances. such as neophytadiene were also identified In a trypanocidal assay, fractions BT-H and BT-D showed IC(50) values of 168 and 19 1 mu g/mL. respectively, and were mote active than the gentian violet standard (31 mu g/ml.); subfractions H02. H03, D01 and D02 were active against L amasonensis, exhibiting IC(50) values of 1 S. 2 7, 4 4. and 4 3 mu g/mL., respectively (standard amphotericin B IC(50) = 13 mu g/mL.) All fractions showed antifungal potential this work reports the biological activity and identification of compounds by GC/MS for the marine red alga B tenella for the first time (C) 2010 Elsevier B V All lights reserved (AU)

• Processo FAPESP: 07/57985-8 - Avaliação química e biológica da macroalga vermelha Bostrychia tenella (Rhodomelaceae)
• Beneficiário: Rafael de Felício
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 05/53808-9 - Avaliação química, biológica e biossintética de espécies de macroalgas vermelhas do gênero Bostrychia (Rhodophyta, Rhodomelaceae)
• Beneficiário: Hosana Maria Debonsi
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Jovens Pesquisadores