| Texto completo | |
| Autor(es): |
Cipriano, T. C.
[1, 2]
;
Takahashi, P. M.
[1, 2]
;
de Lima, D.
[1, 2]
;
Oliveira, Jr., V. X.
[1]
;
Souza, J. A.
[1]
;
Martinho, H.
[1]
;
Alves, W. A.
[1, 2]
Número total de Autores: 7
|
| Afiliação do(s) autor(es): | [1] Univ Fed ABC, Ctr Ciencias Nat & Humanas, Santo Andre, SP - Brazil
[2] Inst Nacl Ciencia & Tecnol Bioanalit, BR-13083970 Campinas, SP - Brazil
Número total de Afiliações: 2
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Journal of Materials Science; v. 45, n. 18, p. 5101-5108, SEP 2010. |
| Citações Web of Science: | 35 |
| Resumo | |
A novel biosensor for hydrogen peroxide was developed by combining the known properties of microperoxidase-11 (MP11) as an oxidation catalyst, and the interesting properties of diphenylalanine peptide nanotubes (PNTs) as a supporting matrix to allow a good bioelectrochemical interface. In this case, the synthesized MP11/PNTs were immobilized onto the ITO electrode surface via layer-by-layer (LBL) deposition, using poly(allylamine hydrochloride) (PAH) as positively charged polyelectrolyte layers. The PNTs provide a favorable microenvironment for MP11 to perform direct electron transfer to the electrode surface. The resulting electrodes showed a pair of well-defined redox peaks with formal potential at about -343 mV (versus SCE) in phosphate buffer solution (pH 7). The experimental results also demonstrated that the resulting biosensor exhibited good electrocatalytic activity to the reduction of H(2)O(2) with a sensitivity of 9.43 mu A cm(-2) mmol(-1) L, and a detection limit of 6 mu mol L(-1) at the signal-to-noise ratio of 3. Moreover, we also observed that the peptides self-assembly can be influenced upon changing the pH of the solution. Alkaline solution appears to favor the packing of diphenylalanine nanotubes being closer than acidic or neutral conditions. The study proved that the combination of PNTs with MP11 is able to open new opportunities for the design of enzymatic biosensors with potential applications in practice. (AU) | |
| Processo FAPESP: | 08/51074-6 - Auto-organizacao de peptideos para construcao de nanotubos e o estudo de suas propriedades estruturais e fisico-quimicas. |
| Beneficiário: | Pedro Mitsuo Takahashi |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |
| Processo FAPESP: | 05/53241-9 - Estudo de fenômenos intergranulares em materiais cerâmicos |
| Beneficiário: | Reginaldo Muccillo |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 08/55549-9 - Imobilizacao de sistemas biomimeticos em nanoestruturas peptidicas aplicadas ao desenvolvimento de biossensores. |
| Beneficiário: | Thiago de Carvalho Cipriano |
| Modalidade de apoio: | Bolsas no Brasil - Mestrado |
| Processo FAPESP: | 08/53576-9 - Síntese, caracterização e estudo das propriedades eletrônicas dos nanotubos de peptídeos e óxido de titânio |
| Beneficiário: | Wendel Andrade Alves |
| Modalidade de apoio: | Auxílio à Pesquisa - Jovens Pesquisadores |
| Processo FAPESP: | 08/57805-2 - Instituto de Bioanalítica |
| Beneficiário: | Lauro Tatsuo Kubota |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |