Micromorphology of Resin-Dentin Interfaces Using One-Bottle Etch&Rinse and Self-Etching Adhesive Systems on Laser-Treated Dentin Surfaces: A Confocal Laser Scanning Microscope Analysis

Autor(es)

de Oliveira, Marcelo Tavares ^[1] ; Galvao Arrais, Cesar Augusto ^[2] ; Aranha, Ana Cecilia ^[3] ; Eduardo, Carlos de Paula ^[3] ; Miyake, Katsuya ^[4] ; Rueggeberg, Frederick Allen ^[5] ; Giannini, Marcelo ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Dept Restorat Dent, Piracicaba Dent Sch, BR-13414903 Piracicaba, SP - Brazil

^[2] Univ Guarulhos, Dept Operat Dent, Sch Dent, BR-07011040 Guarulhos, SP - Brazil

^[3] Univ Sao Paulo, Dept Restorat Dent, Sch Dent, BR-05508900 Sao Paulo - Brazil

^[4] Kagawa Univ, Dept Histol & Cell Biol, Sch Med, Kagawa 7610793 - Japan

^[5] Med Coll Georgia, Dent Mat Sect, Dept Oral Rehabil, Sch Dent, Augusta, GA 30912 - USA

• Tipo de documento: Artigo Científico
• Fonte: Lasers in Surgery and Medicine; v. 42, n. 7, p. 662-670, SEP 2010.
• Citações Web of Science: 14

Resumo

Background and Objectives: This study evaluated the hybrid layer (HL) morphology created by three adhesive systems (AS) on dentin surfaces treated with Er:YAG laser using two irradiation parameters. Study Design: Occlusal flat dentin surfaces of 36 human third molars were assigned into nine groups (n = 4) according to the following ASs: one bottle etch\&rinse Single Bond Plus (3M ESPE), two-step Clearfil Protect Bond (Kuraray), and all-in-one S3 Bond (Kuraray) self-etching, which were labeled with rhodamine B or fluorescein isothiocyanate dextran and were applied to dentin surfaces that were irradiated with Er:YAG laser at either 120 (38.7 J/cm(2)) or 200 mJ/pulse (64.5 J/cm(2)), or were applied to untreated dentin surfaces (control group). The ASs were light-activated following MI and the bonded surfaces were restored with resin composite Z250 (3M ESPE). After 24 hours of storage in vegetable oil, the restored teeth were vertically, serially sectioned into 1-mm thick slabs, which had the adhesive interfaces analyzed with confocal laser microscope (CLSM-LSM 510 Meta). CLSM images were recorded in the fluorescent mode from three different regions along each bonded interface. Results: Non-uniform HL was created on laser-irradiated dentin surfaces regardless of laser irradiation protocol for all AS, while regular and uniform HL was observed in the control groups. ``Stretch mark{''}-like red lines were found within the HL as a result of resin infiltration into dentin microfissures, which were predominantly observed in 200 mJ/pulse groups regardless of AS. Poor resin infiltration into peritubular dentin was observed in most regions of adhesive interfaces created by all ASs on laser-irradiated dentin, resulting in thin resin tags with neither funnel-shaped morphology nor lateral resin projections. Conclusion: Laser irradiation of dentin surfaces at 120 or 200 mJ/pulse resulted in morphological changes in HL and resin tags for all ASs evaluated in the study. Lasers Surg. Med. 42:662-670, 2010. (C) 2010 Wiley-Liss, Inc. (AU)

• Processo FAPESP: 05/56533-0 - Avaliacao dos efeitos da irradiacao do substrato dentinario com laser er:yag nos procedimentos restauradores adesivos: analise da permeabilidade, das propriedades fisicas e observacao microscopica.
• Beneficiário: Marcelo Tavares de Oliveira
• Modalidade de apoio: Bolsas no Brasil - Doutorado