Effect of Isolated Fractions of Harpagophytum procumbens DC (Devil's Claw) on COX-1, COX-2 Activity and Nitric Oxide Production on Whole-Blood Assay

Autor(es)

Anauate, Maria Cecilia ^[1] ; Torres, Luce Maria ^[2] ; Verissimo de Mello, Suzana Beatriz ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Med, Dept Internal Med, Div Rheumatol, BR-01246903 Sao Paulo - Brazil

^[2] Inst Bot, Dept Plant Physiol & Biochem, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Phytotherapy Research; v. 24, n. 9, p. 1365-1369, SEP 2010.
• Citações Web of Science: 17

Resumo

The present study evaluates the effect of isolated fractions of Harpagophytum procumbens (devil's claw) on cyclooxygenase (COX-1 and COX-2) activities and NO production using a whole blood assay. The activity of COX-1 was quantified as platelet thromboxane B(2) production in blood clotting and COX-2 as prostaglandin E(2) production in LPS-stimulated whole blood. Total NO(2)(-)/NO(3)(-) concentration was determined by Griess reaction in LPS stimulated blood. Assays were performed by incubation of isolated fractions obtained by flash chromatography monitored with HPLC, TLC and identified by (1)HNMR, containing different amounts of harpagoside with blood from healthy donors. Indomethacin and etoricoxib were the positive controls of COX-1 and COX-2 Inhibition. Data shows that fraction containing the highest concentration of harpagoside inhibited indistinctively COX-1 and COX-2 (37.2 and 29.5% respectively) activity and greatly inhibited NO production (66%). In contrast the fraction including iridoid pool increased COX-2 and did not alter NO and COX-1 activities. The fraction containing cinnamic acid was able to reduce only NO production (67%). Our results demonstrated that the harpagoside fraction is the main responsible for the effect of devils claw on these enzyme activities. However, other components from devil's claw crude extract could antagonize or increase the synthesis of inflammatory mediators. Copyright (C) 2010 John Wiley \& Sons, Ltd. (AU)

• Processo FAPESP: 04/02452-7 - Estudo in vitro e ex vivo da atividade de flavonoides e de extratos de uncaria tomentosa e harpagophytum procumbens sobre a atividade de cox-1 e cox-2 e inos em ensaios de sangue total.
• Beneficiário: Suzana Beatriz Verissimo de Mello
• Modalidade de apoio: Auxílio à Pesquisa - Regular