Substantivity of chlorhexidine to human dentin

Autor(es)

Carrilho, Marcela R. ^{[1, 2]} ; Carvalho, Ricardo M. ^{[3, 4]} ; Sousa, Ethan N. ^[5] ; Nicolau, Jose ^[5] ; Breschi, Lorenzo ^{[6, 7]} ; Mazzoni, Annalisa ^[8] ; Tjaderhane, Leo ^{[9, 10]} ; Tay, Franklin R. ^[11] ; Agee, Kelli ^[11] ; Pashley, David H. ^[11]

Afiliação do(s) autor(es):Mostrar menos -

^[1] Univ Estadual Campinas, Piracicaba Dent Sch, Piracicaba - Brazil

^[2] Univ Bandeirante Sao Paulo, Hlth Inst, GEO UNIBAN, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Bauru Sch Dent, Dept Prosthodont, Bauru - Brazil

^[4] Univ Florida, Coll Dent, Dept Operat Dent, Gainesville, FL - USA

^[5] Univ Sao Paulo, Sch Dent, Dent Mat Dept, Oral Biol Res Ctr, Sao Paulo - Brazil

^[6] Univ Trieste, Dept Biomed, Trieste - Italy

^[7] IGM CNR, Unit Bologna, IOR, Bologna - Italy

^[8] Univ Bologna, Dept SAU&FAL, Bologna - Italy

^[9] Univ Oulu, Inst Dent, Oulu - Finland

^[10] Oulu Univ Hosp, Oulu - Finland

^[11] Med Coll Georgia, Sch Dent, Dept Oral Biol, Augusta, GA 30912 - USA

• Tipo de documento: Artigo Científico
• Fonte: Dental Materials; v. 26, n. 8, p. 779-785, AUG 2010.
• Citações Web of Science: 96

Resumo

Objectives. To better comprehend the role of CHX in the preservation of resin-dentin bonds, this study investigated the substantivity of CHX to human dentin. Material and methods. Dentin disks (n = 45) were obtained from the mid-coronal portion of human third molars. One-third of dentin disks were kept mineralized (MD), while the other two-thirds had one of the surfaces partially demineralized with 37% phosphoric acid for 15 s (PDD) or they were totally demineralized with 10% phosphoric acid (TDD). Disks of hydroxyapatite (HA) were also prepared. Specimens were treated with: (1) 10 mu L of distilled water (controls), (2) 10 mu L of 0.2% chlorhexidine diacetate (0.2% CHX) or (3) 10 mu L of 2% chlorhexidine diacetate (2% CHX). Then, they were incubated in 1 mL of PBS (pH 7.4, 37 degrees C). Substantivity was evaluated as a function of the CHX-applied dose after: 0.5 h, 1 h, 3 h, 6 h, 24 h, 168 h (1 week), 672 h (4 weeks) and 1344 h (8 weeks) of incubation. CHX concentration in eluates was spectrophotometrically analyzed at 260 nm. Results. Significant amounts of CHX remained retained in dentin substrates (MD, PPD or TDD), independent on the CHX-applied dose or time of incubation (p < 0.05). High amounts of retained CHX onto HA were observed only for specimens treated with the highest concentration of CHX (2%) (p < 0.05). Conclusion. The outstanding substantivity of CHX to dentin and its reported effect on the inhibition of dentinal proteases may explain why CHX can prolong the durability of resin-dentin bonds. (C) 2010 Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 07/54618-4 - Avaliação da atividade de colagenases na degradação da dentina humana tratada por procedimentos restauradores adesivos
• Beneficiário: Marcela Rocha de Oliveira Carrilho
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores