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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Substantivity of chlorhexidine to human dentin

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Author(s):
Carrilho, Marcela R. [1, 2] ; Carvalho, Ricardo M. [3, 4] ; Sousa, Ethan N. [5] ; Nicolau, Jose [5] ; Breschi, Lorenzo [6, 7] ; Mazzoni, Annalisa [8] ; Tjaderhane, Leo [9, 10] ; Tay, Franklin R. [11] ; Agee, Kelli [11] ; Pashley, David H. [11]
Total Authors: 10
Affiliation:
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[1] Univ Estadual Campinas, Piracicaba Dent Sch, Piracicaba - Brazil
[2] Univ Bandeirante Sao Paulo, Hlth Inst, GEO UNIBAN, Sao Paulo - Brazil
[3] Univ Sao Paulo, Bauru Sch Dent, Dept Prosthodont, Bauru - Brazil
[4] Univ Florida, Coll Dent, Dept Operat Dent, Gainesville, FL - USA
[5] Univ Sao Paulo, Sch Dent, Dent Mat Dept, Oral Biol Res Ctr, Sao Paulo - Brazil
[6] Univ Trieste, Dept Biomed, Trieste - Italy
[7] IGM CNR, Unit Bologna, IOR, Bologna - Italy
[8] Univ Bologna, Dept SAU&FAL, Bologna - Italy
[9] Univ Oulu, Inst Dent, Oulu - Finland
[10] Oulu Univ Hosp, Oulu - Finland
[11] Med Coll Georgia, Sch Dent, Dept Oral Biol, Augusta, GA 30912 - USA
Total Affiliations: 11
Document type: Journal article
Source: Dental Materials; v. 26, n. 8, p. 779-785, AUG 2010.
Web of Science Citations: 96
Abstract

Objectives. To better comprehend the role of CHX in the preservation of resin-dentin bonds, this study investigated the substantivity of CHX to human dentin. Material and methods. Dentin disks (n = 45) were obtained from the mid-coronal portion of human third molars. One-third of dentin disks were kept mineralized (MD), while the other two-thirds had one of the surfaces partially demineralized with 37% phosphoric acid for 15 s (PDD) or they were totally demineralized with 10% phosphoric acid (TDD). Disks of hydroxyapatite (HA) were also prepared. Specimens were treated with: (1) 10 mu L of distilled water (controls), (2) 10 mu L of 0.2% chlorhexidine diacetate (0.2% CHX) or (3) 10 mu L of 2% chlorhexidine diacetate (2% CHX). Then, they were incubated in 1 mL of PBS (pH 7.4, 37 degrees C). Substantivity was evaluated as a function of the CHX-applied dose after: 0.5 h, 1 h, 3 h, 6 h, 24 h, 168 h (1 week), 672 h (4 weeks) and 1344 h (8 weeks) of incubation. CHX concentration in eluates was spectrophotometrically analyzed at 260 nm. Results. Significant amounts of CHX remained retained in dentin substrates (MD, PPD or TDD), independent on the CHX-applied dose or time of incubation (p < 0.05). High amounts of retained CHX onto HA were observed only for specimens treated with the highest concentration of CHX (2%) (p < 0.05). Conclusion. The outstanding substantivity of CHX to dentin and its reported effect on the inhibition of dentinal proteases may explain why CHX can prolong the durability of resin-dentin bonds. (C) 2010 Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved. (AU)