Increased Viability of Odontoblast-Like Cells Subjected to Low-Level Laser Irradiation

Autor(es)

Oliveira, C. F. ^[1] ; Basso, F. G. ^[2] ; Lins, E. C. ^[3] ; Kurachi, C. ^[3] ; Hebling, J. ^[1] ; Bagnato, V. S. ^[3] ; de Souza Costa, C. A. ^[1]

Afiliação do(s) autor(es)

^[1] UNESP Univ Estadual Paulista, Araraquara Sch Dent, BR-14801903 Araraquara, SP - Brazil

^[2] UNICAMP Univ Campinas, Piracicaba Sch Dent, BR-13414903 Piracicaba, SP - Brazil

^[3] Univ Sao Paulo, Phys Inst Sao Carlos, BR-13560970 Sao Carlos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Laser Physics; v. 20, n. 7, p. 1659-1666, JUL 2010.
• Citações Web of Science: 30

Resumo

Studies have shown that the increase of cell metabolism depends on the low level laser therapy (LLLT) parameters used to irradiate the cells. However, the optimal laser dose to up-regulate pulp cell activity remains unknown. Consequently, the aim of this study was to evaluate the metabolic response of odontoblast-like cells (MDPC-23) exposed to different LLLT doses. Cells at 20000 cells/cm(2) were seeded in 24-well plates using plain culture medium (DMEM) and were incubated in a humidified incubator with 5% CO(2) at 37 degrees C. After 24 h, the culture medium was replaced by fresh DMEM supplemented with 5% (stress by nutritional deficit) or 10% fetal bovine serum (FBS). The cells were exposed to different laser doses from a near infrared diode laser prototype designed to provide a uniform irradiation of the wells. The experimental groups were: G1: 1.5 J/cm(2) + 5% FBS; G2: 1.5 J/cm(2) + 10% FBS; G3: 5 J/cm(2) + 5% FBS; G4: 5 J/cm(2) + 10% FBS; G5: 19 J/cm(2) + 5% FBS; G6: 19 J/cm(2) + 10% FBS. LLLT was performed in 3 consecutive irradiation cycles with a 24-hour interval. Non-irradiated cells cultured in DMEM supplemented with either 5 or 10% FBS served as control groups. The analysis of the metabolic response was performed by the MTT assay 3 h after the last irradiation. G1 presented an increase in SDH enzyme activity and differed significantly (Mann-Whitney test, p < 0.05) from the other groups. Analysis by scanning electron microscopy showed normal cell morphology in all groups. Under the tested conditions, LLLT stimulated the metabolic activity of MDPC-23 cultured in DMEM supplemented with 5% FBS and exposed to a laser dose of 1.5 J/cm(2). These findings are relevant for further studies on the action of near infrared lasers on cells with odontoblast phenotype. (AU)

• Processo FAPESP: 08/54785-0 - Efeito do laser de baixa intensidade sobre células odontoblastoides in vitro e complexo dentino-pulpar in vivo
• Beneficiário: Camila Fávero de Oliveira
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 07/50646-3 - Avaliação do efeito bioestimulador direto e transdentinário do laser de baixa potência sobre células odontoblastóides em cultura
• Beneficiário: Carlos Alberto de Souza Costa
• Modalidade de apoio: Auxílio à Pesquisa - Regular