| Texto completo | |
| Autor(es): |
Oliveira, C. F.
[1]
;
Basso, F. G.
[2]
;
Lins, E. C.
[3]
;
Kurachi, C.
[3]
;
Hebling, J.
[1]
;
Bagnato, V. S.
[3]
;
de Souza Costa, C. A.
[1]
Número total de Autores: 7
|
| Afiliação do(s) autor(es): | [1] UNESP Univ Estadual Paulista, Araraquara Sch Dent, BR-14801903 Araraquara, SP - Brazil
[2] UNICAMP Univ Campinas, Piracicaba Sch Dent, BR-13414903 Piracicaba, SP - Brazil
[3] Univ Sao Paulo, Phys Inst Sao Carlos, BR-13560970 Sao Carlos, SP - Brazil
Número total de Afiliações: 3
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Laser Physics; v. 20, n. 7, p. 1659-1666, JUL 2010. |
| Citações Web of Science: | 30 |
| Resumo | |
Studies have shown that the increase of cell metabolism depends on the low level laser therapy (LLLT) parameters used to irradiate the cells. However, the optimal laser dose to up-regulate pulp cell activity remains unknown. Consequently, the aim of this study was to evaluate the metabolic response of odontoblast-like cells (MDPC-23) exposed to different LLLT doses. Cells at 20000 cells/cm(2) were seeded in 24-well plates using plain culture medium (DMEM) and were incubated in a humidified incubator with 5% CO(2) at 37 degrees C. After 24 h, the culture medium was replaced by fresh DMEM supplemented with 5% (stress by nutritional deficit) or 10% fetal bovine serum (FBS). The cells were exposed to different laser doses from a near infrared diode laser prototype designed to provide a uniform irradiation of the wells. The experimental groups were: G1: 1.5 J/cm(2) + 5% FBS; G2: 1.5 J/cm(2) + 10% FBS; G3: 5 J/cm(2) + 5% FBS; G4: 5 J/cm(2) + 10% FBS; G5: 19 J/cm(2) + 5% FBS; G6: 19 J/cm(2) + 10% FBS. LLLT was performed in 3 consecutive irradiation cycles with a 24-hour interval. Non-irradiated cells cultured in DMEM supplemented with either 5 or 10% FBS served as control groups. The analysis of the metabolic response was performed by the MTT assay 3 h after the last irradiation. G1 presented an increase in SDH enzyme activity and differed significantly (Mann-Whitney test, p < 0.05) from the other groups. Analysis by scanning electron microscopy showed normal cell morphology in all groups. Under the tested conditions, LLLT stimulated the metabolic activity of MDPC-23 cultured in DMEM supplemented with 5% FBS and exposed to a laser dose of 1.5 J/cm(2). These findings are relevant for further studies on the action of near infrared lasers on cells with odontoblast phenotype. (AU) | |
| Processo FAPESP: | 08/54785-0 - Efeito do laser de baixa intensidade sobre células odontoblastoides in vitro e complexo dentino-pulpar in vivo |
| Beneficiário: | Camila Fávero de Oliveira |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |
| Processo FAPESP: | 07/50646-3 - Avaliação do efeito bioestimulador direto e transdentinário do laser de baixa potência sobre células odontoblastóides em cultura |
| Beneficiário: | Carlos Alberto de Souza Costa |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |