Histopathology, humoral and cellular immune response in the murine model of Leishmania (Viannia) shawi

Autor(es)

Domingues Passero, Luiz Felipe ^[1] ; Marques, Claudia ^{[2, 3]} ; Vale-Gato, Ines ^{[2, 3]} ; Pereira Corbett, Carlos Eduardo ^[1] ; Laurenti, Marcia Dalastra ^[1] ; Santos-Gomes, Gabriela ^{[2, 3]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Med, Lab Patol Molestias Infecciosas, Sao Paulo - Brazil

^[2] Univ Nova Lisboa, UEI Leishmanioses, Ctr Malaria, Inst Higiene & Med Trop, P-1349008 Lisbon - Portugal

^[3] Univ Nova Lisboa, Inst Higiene & Med Trop, Trop Dis Associated Lab, P-1349008 Lisbon - Portugal

• Tipo de documento: Artigo Científico
• Fonte: Parasitology International; v. 59, n. 2, p. 159-165, JUN 2010.
• Citações Web of Science: 14

Resumo

Leishmania (Viannia) shawl was recently characterized and few studies concerning modifications in cellular and humoral immune responses in experimental leishmaniasis have been conducted. In this work, immunopathological changes induced by L. shawl in chronically infected BALB/c mice were investigated. Infected BALB/c mice developed increased lesion size associated with strong inflammatory infiltrate diffusely distributed in the dermis, with highly infected macrophages. The humoral immune response was predominantly directed toward the IgG1 isotype. The functional activity of CD4(+) and CD8(+) T cells showed significantly increased TNF-alpha mRNA levels associated with reduced IFN-gamma expression by CD4(+) T cells and the double negative (dn) CD4CD8 cell subset. High IL-4 levels expressed by CD8(+) T cells and dnCD4CD8 and TGF-beta by CD4(+) and CD8(+) T cells were detected, while IL-10 was highly expressed by all three cell subpopulations. Taken together, these results show an evident imbalance between TNF-alpha and IFN-gamma that is unfavorable to amastigote replication control. Furthermore, L. shawi seems to regulate different cell populations to express deactivating cytokines to avoid its own destruction. This study indicates BALB/c mice as a potentially good experimental model for further studies on American cutaneous leishmaniosis caused by L. shawi. (C) 2010 Elsevier Ireland Ltd. All rights reserved. (AU)

• Processo FAPESP: 07/50654-6 - Avaliação da resposta imune celular desencadeada por antígenos protéicos isolados de Leishmania (Viannia) shawi
• Beneficiário: Luiz Felipe Domingues Passero
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 07/56209-4 - Avaliacao da pesquisa imune celular desencadeada por antigenos proteicos isolados de leishmania (viannia) shawi.
• Beneficiário: Marcia Dalastra Laurenti
• Modalidade de apoio: Auxílio à Pesquisa - Regular