Genomics and proteomics approaches to the study of cancer-stroma interactions

Autor(es):Mostrar menos -

Rodrigues-Lisoni, Flavia C. ^{[1, 2]} ; Peitl, Jr., Paulo ^[3] ; Vidotto, Alessandra ^[1] ; Polachini, Giovana M. ^[1] ; Maniglia, Jose V. ^[4] ; Carmona-Raphe, Juliana ^[1] ; Cunha, Bianca R. ^[1] ; Henrique, Tiago ^[1] ; Souza, Caique F. ^{[5, 1]} ; Teixeira, Rodrigo A. P. ^[3] ; Fukuyama, Erica E. ^[6] ; Michaluart, Jr., Pedro ^[7] ; de Carvalho, Marcos B. ^[8] ; Oliani, Sonia M. ^[3] ; Tajara, Eloiza H. ^{[5, 1]} ; GENCAPO, Head Neck Genome Project

Afiliação do(s) autor(es)

^[1] Sch Med FAMERP, Dept Mol Biol, Sao Jose Do Rio Preto - Brazil

^[2] UNESP, Fac Engn Ilha Solteria, Dept Biol & Zootechny, Ilha Solteira - Brazil

^[3] Sao Paulo State Univ UNESP, IBILCE, Dept Biol, Sao Jose Do Rio Preto - Brazil

^[4] Sch Med FAMERP, Dept Otorhinolaryngol & Head & Neck Surg, Sao Jose Do Rio Preto - Brazil

^[5] Univ Sao Paulo, Inst Biociencias, Dept Genet & Evolutionary Biol, Sao Paulo - Brazil

^[6] Arnaldo Vieira de Carvalho Hosp, Dept Head & Neck Surg, Sao Paulo - Brazil

^[7] Univ Sao Paulo, Sch Med, Dept Surg, Div Head & Neck Surg, BR-09500900 Sao Paulo - Brazil

^[8] Heliopolis Hosp, Dept Head & Neck Surg, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BMC MEDICAL GENOMICS; v. 3, MAY 4 2010.
• Citações Web of Science: 26

Resumo

Background: The development and progression of cancer depend on its genetic characteristics as well as on the interactions with its microenvironment. Understanding these interactions may contribute to diagnostic and prognostic evaluations and to the development of new cancer therapies. Aiming to investigate potential mechanisms by which the tumor microenvironment might contribute to a cancer phenotype, we evaluated soluble paracrine factors produced by stromal and neoplastic cells which may influence proliferation and gene and protein expression. Methods: The study was carried out on the epithelial cancer cell line (Hep-2) and fibroblasts isolated from a primary oral cancer. We combined a conditioned-medium technique with subtraction hybridization approach, quantitative PCR and proteomics, in order to evaluate gene and protein expression influenced by soluble paracrine factors produced by stromal and neoplastic cells. Results: We observed that conditioned medium from fibroblast cultures (FCM) inhibited proliferation and induced apoptosis in Hep-2 cells. In neoplastic cells, 41 genes and 5 proteins exhibited changes in expression levels in response to FCM and, in fibroblasts, 17 genes and 2 proteins showed down-regulation in response to conditioned medium from Hep-2 cells (HCM). Nine genes were selected and the expression results of 6 down-regulated genes (ARID4A, CALR, GNB2L1, RNF10, SQSTM1, USP9X) were validated by real time PCR. Conclusions: A significant and common denominator in the results was the potential induction of signaling changes associated with immune or inflammatory response in the absence of a specific protein. (AU)

• Processo FAPESP: 04/14846-0 - Rede de proteoma do estado de São Paulo
• Beneficiário: Fabio Cesar Gozzo
• Modalidade de apoio: Auxílio à Pesquisa - Programa GENOMA
• Processo FAPESP: 06/60162-0 - Investigação da interação estroma-tumor em carcinomas de cabeça e pescoço
• Beneficiário: Flávia Cristina Rodrigues Lisoni
• Modalidade de apoio: Auxílio à Pesquisa - Programa Primeiros Projetos
• Processo FAPESP: 04/12054-9 - Busca de marcadores de agressividade em tumores de cabeça e pescoço
• Beneficiário: Eloiza Helena Tajara da Silva
• Modalidade de apoio: Auxílio à Pesquisa - Temático