Evaluation of rhBMP-2 and Natural Latex as Potential Osteogenic Proteins in Critical Size Defects by Histomorphometric Methods

Autor(es)

Mardegan Issa, Joao Paulo ^[1] ; Aparecido Defino, Helton Luiz ^[2] ; Netto, Joaquim Coutinho ^[3] ; Volpon, Jose Batista ^[2] ; Hallak Regalo, Simone Cecilio ; Iyomasa, Mamie Mizusaki ; Siessere, Selma ; Tiossi, Rodrigo ^[4]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Odontol Ribeirao Preto, Dept Morphol Stomathol & Physiol, Sch Dent Ribeirao Preto, BR-14040904 Ribeirao Preto - Brazil

^[2] Univ Sao Paulo, Dept Biomech Rehabil & Med Locomotor Apparatus, Sch Med Ribeirao Preto, BR-14040904 Ribeirao Preto - Brazil

^[3] Univ Sao Paulo, Dept Immunol & Biochem, Sch Med Ribeirao Preto, BR-14040904 Ribeirao Preto - Brazil

^[4] Univ Sao Paulo, Dept Dent Mat & Prostheses, Sch Dent Ribeirao Preto, BR-14040904 Ribeirao Preto - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Anatomical Record-Advances in Integrative Anatomy and Evolutionary Biology; v. 293, n. 5, p. 794-801, MAY 2010.
• Citações Web of Science: 18

Resumo

This in vivo study evaluated the osteogenic potential of two proteins, recombinant human bone morphogenetic protein-2 (rhBMP-2) and a protein extracted from natural latex (Hevea brasiliensis, P-1), and compared their effects on bone defects when combined with a carrier or a collagen gelatin. Eighty-four (84) Wistar rats were divided into two groups, with and without the use of collagen gelatin, and each of these were divided into six treatment groups of seven animals each. The treatment groups were: (1) 5 mu g of pure rhBMP-2; (2) 5 mu g of rhBMP-2/monoolein gel; (3) pure monoolein gel; (4) 5 mu g of pure P-1; (5) 5 mu g of P-1/monoolein gel; (6) critical bone defect control. The animals were anesthetized and a 6 mm diameter critical bone defect was made in the left posterior region of the parietal bone. Animals were submitted to intracardiac perfusion after 4 weeks and the calvaria tissue was removed for histomorphometric analysis. In this experimental study, it was concluded that rhBMP-2 allowed greater new bone formation than P-1 protein and this process was more effective when the bone defect was covered with collagen gelatin (P < 0.05). Anat Rec, 293:794-801, 2010. (C) 2010 Wiley-Liss, Inc. (AU)

• Processo FAPESP: 07/59465-1 - Avaliação comparativa entre as proteínas osteoindutoras rhBMP-2 e a proteína extraída da seringueira Hevea brasiliensis no reparo de defeitos ósseos críticos realizados em calvárias de ratos
• Beneficiário: João Paulo Mardegan Issa
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado