Protective effect of rosmarinic acid on V79 cells evaluated by the micronucleus and comet assays

Autor(es)

Furtado, Ricardo Andrade ^[1] ; Rezende de Araujo, Felipe Rodrigues ^[1] ; Resende, Flavia Aparecida ^[1] ; Cunha, Wilson Roberto ^[1] ; Tavares, Denise Crispim ^[1]

Afiliação do(s) autor(es)

^[1] Univ Franca, BR-14404600 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF APPLIED TOXICOLOGY; v. 30, n. 3, p. 254-259, APR 2010.
• Citações Web of Science: 29

Resumo

Rosmarinic acid (RA) is a naturally occurring phenolic compound, which contributes to the beneficial and health-promoting effects of herbs, spices and medicinal plants. RA has shown several biological activities, such as hepatoprotective, anti-inflammatory, antiangiogenic, antitumor, antidepressant, antineurodegenerative, HIV-1 inhibitory and antioxidant effects. The aim of this study was to investigate the ability of RA to prevent chemically induced chromosome breakage or loss and primary DNA damage using the micronucleus and comet assays with V79 cells, respectively. The chemotherapeutic agent doxorubicin (DXR; 0.5 mu g ml(-1)) was used as the DNA-damaging agent. The cultures were treated with different concentrations of RA (0.28, 0.56 and 1.12 mM) alone or in combination with DXR. The results showed that RA exerted no genotoxic effect, but significantly reduced the frequency of micronuclei and the extent of DNA damage induced by DXR at the three concentrations tested. The antioxidant activity of RA might be involved in the reduction of DXR-induced DNA damage observed in the present study. Copyright (C) 2009 John Wiley \& Sons, Ltd. (AU)

• Processo FAPESP: 09/00604-8 - Moléculas de interesse: isolamento, identificação, modificações estruturais e avaliação em diversos ensaios biológicos
• Beneficiário: Wilson Roberto Cunha
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 07/07211-6 - Estudo da atividade mutagênica e/ou antimutagênica da Baccharis dracunculifolia e do Artepelin C em células de mamíferos
• Beneficiário: Denise Crispim Tavares Barbosa
• Modalidade de apoio: Auxílio à Pesquisa - Regular