| Texto completo | |
| Autor(es): Mostrar menos - |
Marques, Lucas Miranda
[1, 2]
;
Ueno, Priscilla M.
[1]
;
Buzinhani, Melissa
[1]
;
Cortez, Beatriz A.
[3]
;
Neto, Renata L.
[1]
;
Yamaguti, Mauricio
[1]
;
Oliveira, Rosangela C.
[1]
;
Guimaraes, Ana Marcia S.
[1]
;
Monezi, Telma A.
[1]
;
Braga, Jr., Antonio Carlos R.
[2]
;
Machado-Santelli, Glaucia M.
[3]
;
Timenetsky, Jorge
[1]
Número total de Autores: 12
|
| Afiliação do(s) autor(es): | [1] Univ Sao Paulo, Inst Ciencias Biomed, Dept Microbiol, BR-05508900 Sao Paulo - Brazil
[2] Univ Fed Bahia, Inst Multidisciplinar Saude, Nucleo Tecnol Saude, BR-45055090 Vitoria Da Conquista, BA - Brazil
[3] Univ Sao Paulo, Inst Ciencias Biomed, Dept Biol Celular & Desenvolvimento, BR-05508900 Sao Paulo - Brazil
Número total de Afiliações: 3
|
| Tipo de documento: | Artigo Científico |
| Fonte: | BMC Microbiology; v. 10, MAR 17 2010. |
| Citações Web of Science: | 13 |
| Resumo | |
Background: Understanding mollicutes is challenging due to their variety and relationship with host cells. Invasion has explained issues related to their opportunistic role. Few studies have been done on the Ureaplasma diversum mollicute, which is detected in healthy or diseased bovine. The invasion in Hep-2 cells of four clinical isolates and two reference strains of their ureaplasma was studied by Confocal Laser Scanning Microscopy and gentamicin invasion assay. Results: The isolates and strains used were detected inside the cells after infection of one minute without difference in the arrangement for adhesion and invasion. The adhesion was scattered throughout the cells, and after three hours, the invasion of the ureaplasmas surrounded the nuclear region but were not observed inside the nuclei. The gentamicin invasion assay detected that 1% of the ATCC strains were inside the infected Hep-2 cells in contrast to 10% to the clinical isolates. A high level of phospholipase C activity was also detected in all studied ureaplasma. Conclusions: The results presented herein will help better understand U. diversum infections, aswell as cellular attachment and virulence. (AU) | |
| Processo FAPESP: | 06/56855-0 - Estudo da variabilidade genetica e dos fatores de virulencia de isolados de ureaplasmas de bovinos, caprinos e ovinos. |
| Beneficiário: | Jorge Timenetsky |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |