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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Invasion of Ureaplasma diversum in Hep-2 cells

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Author(s):
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Marques, Lucas Miranda [1, 2] ; Ueno, Priscilla M. [1] ; Buzinhani, Melissa [1] ; Cortez, Beatriz A. [3] ; Neto, Renata L. [1] ; Yamaguti, Mauricio [1] ; Oliveira, Rosangela C. [1] ; Guimaraes, Ana Marcia S. [1] ; Monezi, Telma A. [1] ; Braga, Jr., Antonio Carlos R. [2] ; Machado-Santelli, Glaucia M. [3] ; Timenetsky, Jorge [1]
Total Authors: 12
Affiliation:
[1] Univ Sao Paulo, Inst Ciencias Biomed, Dept Microbiol, BR-05508900 Sao Paulo - Brazil
[2] Univ Fed Bahia, Inst Multidisciplinar Saude, Nucleo Tecnol Saude, BR-45055090 Vitoria Da Conquista, BA - Brazil
[3] Univ Sao Paulo, Inst Ciencias Biomed, Dept Biol Celular & Desenvolvimento, BR-05508900 Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Source: BMC Microbiology; v. 10, MAR 17 2010.
Web of Science Citations: 13
Abstract

Background: Understanding mollicutes is challenging due to their variety and relationship with host cells. Invasion has explained issues related to their opportunistic role. Few studies have been done on the Ureaplasma diversum mollicute, which is detected in healthy or diseased bovine. The invasion in Hep-2 cells of four clinical isolates and two reference strains of their ureaplasma was studied by Confocal Laser Scanning Microscopy and gentamicin invasion assay. Results: The isolates and strains used were detected inside the cells after infection of one minute without difference in the arrangement for adhesion and invasion. The adhesion was scattered throughout the cells, and after three hours, the invasion of the ureaplasmas surrounded the nuclear region but were not observed inside the nuclei. The gentamicin invasion assay detected that 1% of the ATCC strains were inside the infected Hep-2 cells in contrast to 10% to the clinical isolates. A high level of phospholipase C activity was also detected in all studied ureaplasma. Conclusions: The results presented herein will help better understand U. diversum infections, aswell as cellular attachment and virulence. (AU)