FGF-2, TGF beta-1, PDGF-A and respective receptors expression in pleomorphic adenoma myoepithelial cells: an in vivo and in vitro study

Autor(es)

Miguita, Lucyene ^[1] ; Martinez, Elizabeth Ferreira ^[1] ; de Araujo, Ney Soares ^[1] ; de Araujo, Vera Cavalcanti ^[1]

Afiliação do(s) autor(es)

^[1] Sao Leopoldo Mandic Inst & Res Ctr, Dept Oral Pathol, Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Applied Oral Science; v. 18, n. 1, p. 83-91, JAN-FEB 2010.
• Citações Web of Science: 17

Resumo

Myoepithelial cells have an important role in salivary gland tumor development, contributing to a low grade of aggressiveness of these tumors. Normal myoepithelial cells are known by their suppressor function presenting increased expression of extracellular matrix genes and protease inhibitors. The importance of stromal cells and growth factors during tumor initiation and progression has been highlighted by recent literature. Many tumors result from the alteration of paracrine growth factors pathways. Growth factors mediate a wide variety of biological processes such as development, tissue repair and tumorigenesis, and also contribute to cellular proliferation and transformation in neoplastic cells. Objectives: This study evaluated the expression of fibroblast growth factor-2 (FGF-2), transforming growth factor beta-1 (TGF beta-1), platelet-derived growth factor-A (PDGF-A) and their respective receptors (FGFR-1, FGFR-2, TGF beta R-II and PDGFR-alpha) in myoepithelial cells from pleomorphic adenomas (PA) by in vivo and in vitro experiments. Material and Methods: Serial sections were obtained from paraffin-embedded PA samples obtained from the school's files. Myoepithelial cells were obtained from explants of PA tumors provided by surgery from different donors. Immunohistochemistry, cell culture and immunofluorescence assays were used to evaluate growth factor expression. Results: The present findings demonstrated that myoepithelial cells from PA were mainly positive to FGF-2 and FGFR-1 by immunohistochemistry and immunofluorescence. PDGF-A and PDGFR-alpha had moderate expression by immunohistochemistry and presented punctated deposits throughout cytoplasm of myoepithelial cells. FGFR-2, TGF beta-1 and TGF beta R-II were negative in all samples. Conclusions: These data suggested that FGF-2 compared to the other studied growth factors has an important role in PA benign myoepithelial cells, probably contributing to proliferation of these cells through the FGFR-1. (AU)

• Processo FAPESP: 04/07960-0 - Estudo das alterações ocorridas no adenoma durante o processo de malignização em carcinoma ex-adenoma pleomórfico
• Beneficiário: Vera Cavalcanti de Araujo
• Modalidade de apoio: Auxílio à Pesquisa - Temático