In vitro schistosomicidal effects of some phloroglucinol derivatives from Dryopteris species against Schistosoma mansoni adult worms

Autor(es)

Magalhaes, Lizandra G. ^[1] ; Kapadia, Govind J. ^[2] ; da Silva Tonuci, Ligia R. ^[3] ; Caixeta, Soraya C. ^[3] ; Parreira, Natallia A. ^[3] ; Rodrigues, Vanderlei ^[1] ; Da Silva Filho, Ademar A. ^[3]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Bioquim & Imunol, Fac Med Ribeirao Preto, BR-14049900 Ribeirao Preto, SP - Brazil

^[2] Howard Univ, Dept Pharmaceut Sci, Sch Pharm, Washington, DC 20059 - USA

^[3] Univ Franca, Grp Pesquisas Prod Nat, Nucleo Pesquisas Ciencias Exatas & Tecnol, BR-14404600 Franca, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Parasitology Research; v. 106, n. 2, p. 395-401, JAN 2010.
• Citações Web of Science: 70

Resumo

The rhizomes of Dryopteris species have popularly been used as vermifuge in flatworm infections. The aim of this work was to evaluate the in vitro schistosomicidal activity of some phloroglucinol compounds, obtained from the rhizomes of Dryopteris species, against Schistosoma mansoni adult worms. All worm pairs were dead after 24 h of incubation with aspidin 25 to 100 mu M (1), flavaspidic acid 50 and 100 mu M (2), methylene-bis-aspidinol 100 mu M (3), and desaspidin 25 to 100 mu M (4). Worms incubated with 1 (25 to 100 mu M) and 2 (50 to 100 mu M) showed decrease motor activity with tegumental alterations, while 3 (100 mu M) and 4 (10 to 100 mu M) showed decrease motor activity without tegumental alterations. Desaspidinol (5) and filicinic acid (6), at the tested concentrations (10 to 100 mu M), did not show activity against adult worms of S. mansoni. Praziquantel (10 mu M), used as positive control, caused death of the parasites and tegumental alterations without separation of worms. In the groups treated with 100 A mu M of compounds 1-4, the viability of the adult worms was similar to the positive control group, in which the worms were dead. Also, both the egg productions and the development of eggs produced by the adult worms were inhibited by the incubation with compounds 1-4 (10 and 100 mu M) in comparison with the negative control (RPMI 1640 medium). It is suggested that the in vitro schistosomicidal effects of phloroglucinols derivatives 1, 2, 3, and 4 may be related to the inhibition of oxidative phosphorylation pathway in S. mansoni. The present results confirmed the traditional indications of rhizomes from Dryopteris species, which possess phloroglucinol compounds, in the treatment of tapeworm infections. (AU)

• Processo FAPESP: 06/60132-4 - Investigação das atividades esquistossomicida, tripanocida e leishmanicida de lignanas e neolignanas obtidas por acoplamento oxidativo in vitro de fenilpropanóides
• Beneficiário: Ademar Alves da Silva Filho
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 09/05478-0 - Avaliação in vitro da citotoxicidade e da atividade esquistossomicida de algumas naftaquinonas sintéticas.
• Beneficiário: Natallia Alle Parreira
• Modalidade de apoio: Bolsas no Brasil - Programa Capacitação - Treinamento Técnico
• Processo FAPESP: 09/05701-1 - Avaliação in vitro da atividade tripanocida e leishmanicida de algumas naftaquinonas sintéticas.
• Beneficiário: Denis Reis Morais
• Modalidade de apoio: Bolsas no Brasil - Programa Capacitação - Treinamento Técnico