Isolation and characterization of polymorphic microsatellites for the natural populations of barker frog Physalaemus cuvieri

Autor(es)

Conte, M. ^[1] ; Cancado, L. J. ^{[2, 3]} ; Laborda, P. R. ^{[2, 3]} ; Zucchi, M. I. ^[4] ; Andrade, G. V. ^[5] ; Rossa-Feres, D. C. ^[6] ; Siqueira, S. ^[1] ; Souza, A. P. ^{[2, 3]} ; Recco-Pimentel, S. M. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Univ Estadual Campinas, Inst Biol, Dept Biol Celular, BR-13083970 Campinas, SP - Brazil

^[2] Univ Estadual Campinas, Univ Estadual Campinas, Dept Genet, Inst Biol, BR-13083970 Campinas, SP - Brazil

^[3] Univ Estadual Campinas, Univ Estadual Campinas, CBMEG, BR-13083970 Campinas, SP - Brazil

^[4] IAC, Ctr Recursos Genet, Mol Biol Lab, BR-13083970 Campinas, SP - Brazil

^[5] Univ Fed Maranhao, Ctr Ciencias Saude, Dept Biol, BR-65085580 Sao Luis, Maranhao - Brazil

^[6] Univ Estadual Paulista UNESP, Dept Bot & Zool, Inst Biociencias Letras & Ciencias Exatas, BR-15054000 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: CONSERVATION GENETICS; v. 10, n. 6, p. 1849-1852, DEC 2009.
• Citações Web of Science: 2

Resumo

Ten polymorphic microsatellite loci were isolated for Physalaemus cuvieri from a GA-CA enriched library. In 160 P. cuvieri individuals, the number of alleles per locus ranged to 2-9 and the expected heterozygosity ranged from 0.30 to 0.85. The primers were successfully cross-amplified in the congeneric species P. albonotatus, P. ephippifer and Physalaemus cf. cuvieri, suggesting that these loci are potentially useful for studies on population genetic structure of Physalaemus sp. (AU)

• Processo FAPESP: 06/59697-7 - Variabilidade intra- e interpopulacional em Physalaemus cuvieri (Anura, Leptodactylidae): estudo de microssatélites
• Beneficiário: Shirlei Maria Recco-Pimentel
• Modalidade de apoio: Auxílio à Pesquisa - Regular