Molted Feathers as a Source of DNA for Genetic Studies in Waterbird Populations

Autor(es)

Mino, Carolina Isabel ^[1] ; Del Lama, Silvia Nassif ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Carlos, Dept Genet & Evolucao, Lab Genet Aves, BR-13565905 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: WATERBIRDS; v. 32, n. 2, p. 322-329, JUN 2009.
• Citações Web of Science: 11

Resumo

The determination of genetic parameters for most waterbird populations presents considerable challenges because these species frequently breed in inaccessible locations which preclude capture of nesting pairs. The application of non-invasive sampling techniques, such as the collection of molted feathers in the field, could represent an appealing strategy to overcome the above cited problem. Here, the feasibility of sampling molted feathers collected in Brazilian populations of the Roseate Spoonbill (Platalea ajaja; N = 94) and the Jabiru Stork (Jabiru mycteria; N = 10) for genetic studies was evaluated. Each feather yielded high quality DNA with a concentration 1.25 tunes higher than that obtained from feathers from similar sized birds from other groups. Amplification of a 390396 by fragment for molecular sexing and 11 microsatellite loci was successful. The average percentage of positive PCRs (i.e. those that. yielded products) among all Roseate Spoonbill loci and samples was 95.5%. The sampling approach appears suitable to gather genetic information in natural populations of these waterbirds and can be applied to other similar species. (AU)

• Processo FAPESP: 04/15205-8 - Genética de populações de Ciconiiformes (Aves) em áreas úmidas brasileiras
• Beneficiário: Silvia Nassif Del Lama
• Modalidade de apoio: Auxílio à Pesquisa - Regular