Urea amperometric biosensors based on a multifunctional bipolymeric layer: Comparing enzyme immobilization methods

Autor(es)

Massafera, Mariana P. ^[1] ; Cordoba de Torresi, Susana I. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Quim, BR-05513970 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: SENSORS AND ACTUATORS B-CHEMICAL; v. 137, n. 2, p. 476-482, APR 2 2009.
• Citações Web of Science: 17

Resumo

This paper outlines the results obtained with biosensors designed for urea amperometric detection. The incorporation of urease into a bipolymeric substrate consisting of poly(pyrrole) and poly(5-amino-1-naphthol) was performed through four different approaches: direct adsorption, entrapment in cellulose acetate layer. cross-linking with glutaraldehyde, and also covalent attachment to the polymeric matrix. Poly(pyrrole) acts as amperometric transducer in these biosensors, while poly(5-amino-1-naphthol) drastically reduces the interference signal of agents such as ascorbic and uric acids. The biosensors containing urease covalently attached to the substrate provided interesting results in terms of sensitivity towards urea (0.50 mu A cm(-2) mmol(-1) L), lifetime (20 days) and short response times, due to the enzyme immobilization method used. All biosensors analyzed showed also a wide linear concentration range (up to 100 mmol L(-1)) and low detection limits (0.22-0.58 mmol L(-1)). (C) 2009 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 06/53093-2 - Biossensores enzimaticos para deteccao de ureia e outros compostos de interesse clinico.
• Beneficiário: Mariana Pereira Massafera
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 03/10015-3 - Arranjos organizados de macromoléculas e nanoestruturas para o desenvolvimento de sensores e microbaterias
• Beneficiário: Roberto Manuel Torresi
• Modalidade de apoio: Auxílio à Pesquisa - Programa PRONEX - Temático