Gas Chromatographic Analysis of Dimethyltryptamine and beta-Carboline Alkaloids in Ayahuasca, an Amazonian Psychoactive Plant Beverage

Autor(es)

Salum Pires, Ana Paula ^[1] ; Rodrigues De Oliveira, Carolina Dizioli ^[1] ; Moura, Sidnei ^[1] ; Doerr, Felipe Augusto ^[1] ; Silva, Wagner Abreu E. ^[1] ; Yonamine, Mauricio ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Clin & Toxicol Anal, Fac Pharmaceut Sci, BR-05508900 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Phytochemical Analysis; v. 20, n. 2, p. 149-153, MAR-APR 2009.
• Citações Web of Science: 34

Resumo

Introduction - Ayahuasca is obtained by infusing the pounded stems of Banisteriopsis caapi in combination with the leaves of Psychotria viridis. P. viridis is rich in the psychedelic indole N,N-dimethyltryptamine, whereas B. caapi contains substantial amounts of beta-carboline alkaloids, mainly harmine, harmaline and tetrahydroharmine, which are monoamine-oxidase inhibitors. Because of differences in composition in ayahuasca preparations, a method to measure their main active constituents is needed. Objective - To develop a gas chromatographic method for the simultaneous determination of dimethyltryptamine and the main beta-carbolines found in ayahuasca preparations. Methodology - The alkaloids were extracted by means of solid phase extraction (C(18)) and detected by gas chromatography with nitrogen/phosphorous detector. Results - The lower limit of quantification (LLOQ) was 0.02 mg/mL for all analytes. The calibration curves were linear over a concentration range of 0.02-4.0 mg/mL (r(2) > 0.99). The method was also precise (RSD < 10%). Conclusion - A simple gas chromatographic method to determine the main alkaloids found in ayahuasca was developed and validated. The method can be useful to estimate administered doses in animals and humans for further pharmacological and toxicological investigations of ayahuasca. Copyright (C) 2009 John Wiley \& Sons, Ltd. (AU)

• Processo FAPESP: 06/00388-5 - Estudos de farmacocinetica e toxicologia do desenvolvimento dos alcaloides de ayahuasca.
• Beneficiário: Mauricio Yonamine
• Modalidade de apoio: Auxílio à Pesquisa - Regular